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A next-generation proteome array for Schistosoma mansoni.
de Assis, Rafael Ramiro; Ludolf, Fernanda; Nakajima, Rie; Jasinskas, Al; Oliveira, Guilherme C; Felgner, Philip L; Gaze, Soraya T; Loukas, Alex; LoVerde, Philip T; Bethony, Jeffrey M; Correa-Oliveira, Rodrigo; Calzavara-Silva, Carlos E.
Afiliación
  • de Assis RR; Laboratório de Imunologia Celular e Molecular, Instituto Rene Rachou, FIOCRUZ, Belo Horizonte, MG, Brazil; Protein Microarray Laboratory, Division of Infectious Disease, School of Medicine, University of California, Irvine, CA, USA.
  • Ludolf F; Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
  • Nakajima R; Protein Microarray Laboratory, Division of Infectious Disease, School of Medicine, University of California, Irvine, CA, USA.
  • Jasinskas A; Protein Microarray Laboratory, Division of Infectious Disease, School of Medicine, University of California, Irvine, CA, USA.
  • Oliveira GC; Vale Technology Institute of Sustainable Development, Belém, PA, Brazil.
  • Felgner PL; Protein Microarray Laboratory, Division of Infectious Disease, School of Medicine, University of California, Irvine, CA, USA.
  • Gaze ST; Laboratório de Imunologia Celular e Molecular, Instituto Rene Rachou, FIOCRUZ, Belo Horizonte, MG, Brazil.
  • Loukas A; Centre for Biodiscovery and Molecular Development of Therapeutics, Australian Institute of Tropical Health & Medicine, James Cook University, Cairns, QLD, Australia.
  • LoVerde PT; Departments of Biochemistry and Pathology, School of Medicine, University of Texas Health Science Center, 7703 Floyd Curl Dr., San Antonio, TX 78229, USA.
  • Bethony JM; Laboratório de Imunologia Celular e Molecular, Instituto Rene Rachou, FIOCRUZ, Belo Horizonte, MG, Brazil; Department of Microbiology, Immunology, and Tropical Medicine, School of Medicine and Health Science, The George Washington University, Washington, DC, USA. Electronic address: jbethony@gwu.edu
  • Correa-Oliveira R; Laboratório de Imunologia Celular e Molecular, Instituto Rene Rachou, FIOCRUZ, Belo Horizonte, MG, Brazil.
  • Calzavara-Silva CE; Laboratório de Imunologia Celular e Molecular, Instituto Rene Rachou, FIOCRUZ, Belo Horizonte, MG, Brazil; Protein Microarray Laboratory, Division of Infectious Disease, School of Medicine, University of California, Irvine, CA, USA.
Int J Parasitol ; 46(7): 411-5, 2016 06.
Article en En | MEDLINE | ID: mdl-27131510
ABSTRACT
A proteome microarray consisting of 992 Schistosoma mansoni proteins was produced and screened with sera to determine antibody signatures indicative of the clinical stages of schistosomiasis and the identification of subunit vaccine candidates. Herein, we describe the methods used to derive the gene list for this array (representing approximately 10% of the predicted S. mansoni proteome). We also probed a pilot version of the microarray with sera from individuals either acutely or chronically infected with S. mansoni from endemic areas in Brazil and sera from individuals resident outside the endemic area (USA) to determine if the array is functional and informative.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Schistosoma mansoni / Esquistosomiasis mansoni / Proteínas del Helminto / Proteoma / Análisis por Matrices de Proteínas Límite: Animals Idioma: En Revista: Int J Parasitol Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Schistosoma mansoni / Esquistosomiasis mansoni / Proteínas del Helminto / Proteoma / Análisis por Matrices de Proteínas Límite: Animals Idioma: En Revista: Int J Parasitol Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos