Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin.
J Vis Exp
; (110)2016 04 29.
Article
en En
| MEDLINE
| ID: mdl-27168117
ABSTRACT
The hair follicle (HF) is an ideal system for studying the biology and regulation of adult stem cells (SCs). This dynamic mini organ is replenished by distinct pools of SCs, which are located in the permanent portion of the HF, a region known as the bulge. These multipotent bulge SCs were initially identified as slow cycling label retaining cells; however, their isolation has been made feasible after identification of specific cell markers, such as CD34 and keratin 15 (K15). Here, we describe a robust method for isolating bulge SCs and epidermal keratinocytes from mouse HFs utilizing fluorescence activated cell-sorting (FACS) technology. Isolated hair follicle SCs (HFSCs) can be utilized in various in vivo grafting models and are a valuable in vitro model for studying the mechanisms that govern multipotency, quiescence and activation.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Células Madre
/
Queratinocitos
/
Folículo Piloso
Límite:
Animals
Idioma:
En
Revista:
J Vis Exp
Año:
2016
Tipo del documento:
Article