Modular Integrated Secretory System Engineering in Pichia pastoris To Enhance G-Protein Coupled Receptor Expression.

Claes, Katrien; Vandewalle, Kristof; Laukens, Bram; Laeremans, Toon; Vosters, Olivier; Langer, Ingrid; Parmentier, Marc; Steyaert, Jan; Callewaert, Nico

Claes, Katrien; Vandewalle, Kristof; Laukens, Bram; Laeremans, Toon; Vosters, Olivier; Langer, Ingrid; Parmentier, Marc; Steyaert, Jan; Callewaert, Nico.

Afiliación

Claes K; Unit for Medical Biotechnology, Medical Biotechnology Center, VIB , Technologiepark 927, 9052 Ghent, Belgium.
Vandewalle K; Laboratory for Protein Biochemistry and Biomolecular Engineering, Department of Biochemistry and Microbiology, Ghent University , K.L.-Ledeganckstraat 35, 9000 Ghent, Belgium.
Laukens B; Unit for Medical Biotechnology, Medical Biotechnology Center, VIB , Technologiepark 927, 9052 Ghent, Belgium.
Laeremans T; Laboratory for Protein Biochemistry and Biomolecular Engineering, Department of Biochemistry and Microbiology, Ghent University , K.L.-Ledeganckstraat 35, 9000 Ghent, Belgium.
Vosters O; Unit for Medical Biotechnology, Medical Biotechnology Center, VIB , Technologiepark 927, 9052 Ghent, Belgium.
Langer I; Laboratory for Protein Biochemistry and Biomolecular Engineering, Department of Biochemistry and Microbiology, Ghent University , K.L.-Ledeganckstraat 35, 9000 Ghent, Belgium.
Parmentier M; Structural Biology Research Center, VIB , Pleinlaan 2, 1050 Brussels, Belgium.
Steyaert J; Structural Biology Brussels, Vrije Universiteit Brussel , Pleinlaan 2, 1050 Brussels, Belgium.
Callewaert N; Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM) , Campus Erasme, 808 Route de Lennik, B-1070 Brussels, Belgium.

ACS Synth Biol ; 5(10): 1070-1075, 2016 10 21.

Article en En | MEDLINE | ID: mdl-27176489

Asunto(s)

Ingeniería Genética/métodos; Pichia/genética; Receptores Acoplados a Proteínas G/genética; Proteínas Recombinantes/genética; Animales; Células CHO; Camélidos del Nuevo Mundo; Cricetulus; Biblioteca de Genes; Glicosilación; Ingeniería Metabólica/métodos; Pichia/metabolismo; Receptores CXCR4/genética; Receptores CXCR4/metabolismo; Receptores Acoplados a Proteínas G/metabolismo; Proteínas Recombinantes/metabolismo; Anticuerpos de Dominio Único/metabolismo; Respuesta de Proteína Desplegada/genética

Palabras clave

CXCR4; N-glycosylation; Pichia pastoris; membrane protein expression; nanobodies

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Bases de datos: MEDLINE Asunto principal: Pichia / Proteínas Recombinantes / Ingeniería Genética / Receptores Acoplados a Proteínas G Límite: Animals Idioma: En Revista: ACS Synth Biol Año: 2016 Tipo del documento: Article País de afiliación: Bélgica

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