Discovery bioanalysis and in vivo pharmacology as an integrated process: a case study in oncology drug discovery.
Bioanalysis
; 8(14): 1481-98, 2016 Jul.
Article
en En
| MEDLINE
| ID: mdl-27314564
ABSTRACT
BACKGROUND:
A bioanalytical team dedicated to in vivo pharmacology was set up to accelerate the selection and characterization of compounds to be evaluated in animal models in oncology.RESULTS:
A DBS-based serial microsampling procedure was optimized from sample collection to extraction to obtain a generic procedure. UHPLC-high-resolution mass spectrometer configuration allowed for fast quantitative and qualitative analysis. Using an optimized lead compound, we show how bioanalysis supported in vivo pharmacology by generating blood and tumor exposure, drug monitoring and PK/PD data.CONCLUSION:
This process provided unique opportunities for the characterization of drug properties, selection and assessment of compounds in animal models and to support and expedite proof-of-concept studies in oncology.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Leucemia
/
Monitoreo de Drogas
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Descubrimiento de Drogas
/
Pruebas con Sangre Seca
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Antineoplásicos
Tipo de estudio:
Qualitative_research
Límite:
Animals
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Female
/
Humans
Idioma:
En
Revista:
Bioanalysis
Año:
2016
Tipo del documento:
Article
País de afiliación:
Francia