Chemical shift assignments and secondary structure prediction for Q4DY78, a conserved kinetoplastid-specific protein from Trypanosoma cruzi.
Biomol NMR Assign
; 10(2): 325-8, 2016 10.
Article
en En
| MEDLINE
| ID: mdl-27356988
ABSTRACT
Trypanosoma cruzi, Trypanosma brucei and Leishmania spp. are kinetoplastid protozoa causative agents of Chagas disease, sleeping sickness and leishmaniasis, respectively, neglected tropical diseases estimated to infect millions of people worldwide. Their genome sequencing has revealed approximately 50 % of genes encoding hypothetical proteins of unknown function, opening possibilities for novel target identification and drug discovery. Q4DY78 is a putative essential protein from T. cruzi conserved in the related kinetoplastids and divergent from mammalian host proteins. Here we report the (1)H, (15)N, and (13)C chemical shift assignments and secondary structure analysis of the Q4DY78 protein as basis for NMR structure determination, functional analysis and drug screening.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Trypanosoma cruzi
/
Proteínas Protozoarias
/
Secuencia Conservada
/
Resonancia Magnética Nuclear Biomolecular
Tipo de estudio:
Prognostic_studies
/
Risk_factors_studies
Idioma:
En
Revista:
Biomol NMR Assign
Asunto de la revista:
BIOLOGIA MOLECULAR
/
MEDICINA NUCLEAR
Año:
2016
Tipo del documento:
Article
País de afiliación:
Brasil