Metal Fluorides as Analogues for Studies on Phosphoryl Transfer Enzymes.
Angew Chem Int Ed Engl
; 56(15): 4110-4128, 2017 04 03.
Article
en En
| MEDLINE
| ID: mdl-27862756
ABSTRACT
The 1994 structure of a transition-state analogue with AlF4- and GDP complexed to G1α, a small Gâ
protein, heralded a new field of research into the structure and mechanism of enzymes that manipulate the transfer of phosphoryl (PO3- ) groups. The number of enzyme structures in the PDB containing metal fluorides (MFx ) as ligands that imitate either a phosphoryl or a phosphate group was 357 at the end of 2016. They fall into three distinct geometrical classes 1)â
Tetrahedral complexes based on BeF3- that mimic ground-state phosphates; 2)â
octahedral complexes, primarily based on AlF4- , which mimic "in-line" anionic transition states for phosphoryl transfer; and 3)â
trigonal bipyramidal complexes, represented by MgF3- and putative AlF30 moieties, which mimic the geometry of the transition state. The interpretation of these structures provides a deeper mechanistic understanding into the behavior and manipulation of phosphate monoesters in molecular biology. This Review provides a comprehensive overview of these structures, their uses, and their computational development.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Fosfatos
/
Fosfoglicerato Quinasa
/
Compuestos de Aluminio
/
Monoéster Fosfórico Hidrolasas
/
Fluoruros
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Angew Chem Int Ed Engl
Año:
2017
Tipo del documento:
Article
País de afiliación:
Reino Unido