Deletion of conserved sequences in IG-DMR at Dlk1-Gtl2 locus suggests their involvement in expression of paternally expressed genes in mice.
J Reprod Dev
; 63(1): 101-109, 2017 Feb 16.
Article
en En
| MEDLINE
| ID: mdl-27904015
ABSTRACT
Expression regulation of the Dlk1-Dio3 imprinted domain by the intergenic differentially methylated region (IG-DMR) is essential for normal embryonic development in mammals. In this study, we investigated conserved IG-DMR genomic sequences in eutherians to elucidate their role in genomic imprinting of the Dlk1-Dio3 domain. Using a comparative genomics approach, we identified three highly conserved sequences in IG-DMR. To elucidate the functions of these sequences in vivo, we generated mutant mice lacking each of the identified highly conserved sequences using the CRISPR/Cas9 system. Although mutant mice did not exhibit the gross phenotype, deletions of the conserved sequences altered the expression levels of paternally expressed imprinted genes in the mutant embryos without skewing imprinting status. These results suggest that the conserved sequences in IG-DMR are involved in the expression regulation of some of the imprinted genes in the Dlk1-Dio3 domain.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Eliminación de Gen
/
Péptidos y Proteínas de Señalización Intercelular
/
ARN Largo no Codificante
/
Herencia Paterna
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Reprod Dev
Asunto de la revista:
MEDICINA REPRODUTIVA
Año:
2017
Tipo del documento:
Article
País de afiliación:
Japón