Growth hormone modulates in vitro endothelial cell migration and formation of capillary-like structures.
Cell Biol Int
; 41(5): 577-584, 2017 May.
Article
en En
| MEDLINE
| ID: mdl-28205281
ABSTRACT
The generation of new blood vessels is a complex process mediated by a variety of growth factors, and the growth hormone (GH) has been shown to act as a proangiogenic factor. In fact, human GH deficiency or excess are associated with endothelial dysfunction. Moreover, mouse models have revealed the action of GH in both tissue repair and in the microvascular circulation of normal tissues. In this study, we investigated the in vitro effects of GH on endothelial cells. Using a murine endothelioma cell line (tEnd.1), we demonstrated that GH has a mitogenic effect. The hormone also affected the endothelial cellular morphology and augmented the deposition of the extracellular matrix molecules, laminin, and fibronectin, on tEnd.1 surface. GH could stimulate tEnd.1 cell fugetaxis, in transwell chambers migration assay, and increased the formation of capillary-like structures in Matrigel®-coated plates. Given the important role of angiogenesis during tissue injury, for example, at ischemic lesions, these findings shed light on therapeutic angiogenesis, particularly in pathologies where the cardiovascular system has been compromised.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Hormona del Crecimiento
/
Movimiento Celular
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Neovascularización Fisiológica
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Células Endoteliales
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Cell Biol Int
Año:
2017
Tipo del documento:
Article
País de afiliación:
Brasil