Selenium nanoparticles synthesized in aqueous extract of Allium sativum perturbs the structural integrity of Calf thymus DNA through intercalation and groove binding.
Mater Sci Eng C Mater Biol Appl
; 74: 597-608, 2017 May 01.
Article
en En
| MEDLINE
| ID: mdl-28254334
ABSTRACT
Biomedical application of selenium nanoparticles (SeNPs) demands the eco-friendly composite for synthesis of SeNPs. The present study reports an aqueous extract of Allium sativum (AqEAS) plug-up the current need. Modern spectroscopic, microscopic and gravimetric techniques were employed to characterize the synthesized nanoparticles. Characterization studies revealed the formation of crystalline spherical shaped SeNPs. FTIR spectrum brings out the presence of different functional groups in AqEAS, which influence the SeNPs formation and stabilization. Furthermore the different aspects of the interaction between SeNPs and CT-DNA were scrutinized by various spectroscopic and cyclic voltametric studies. The results reveals the intercalation and groove binding mode of interaction of SeNPs with stacked base pair of CT-DNA. The Stern-Volmer quenching constant (KSV) were found to be 7.02×106M-1 (ethidium bromide), 4.22×106 M-1 (acridine orange) and 7.6×106M-1 (Hoechst) indicating strong binding of SeNPs with CT-DNA. The SeNPs - CT-DNA interactions were directly visualized by atomic force microscopy. The present study unveils the cost effective, innocuous, highly stable SeNPs intricate mechanism of DNA interaction, which will be a milestone in DNA targeted chemotherapy.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Selenio
/
Extractos Vegetales
/
Nanopartículas del Metal
/
Ajo
/
Sustancias Intercalantes
Límite:
Animals
Idioma:
En
Revista:
Mater Sci Eng C Mater Biol Appl
Año:
2017
Tipo del documento:
Article
País de afiliación:
India