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Identification and characterization of Laodelphax striatellus (Insecta: Hemiptera: Delphacidae) neutral sphingomyelinase.
Zhou, Y; Lin, X-W; Begum, M-A; Zhang, C-H; Shi, X-X; Jiao, W-J; Zhang, Y-R; Yuan, J-Q; Li, H-Y; Yang, Q; Mao, C; Zhu, Z-R.
Afiliación
  • Zhou Y; State Key Laboratory of Rice Biology; Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects; and Institute of Insect Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
  • Lin XW; Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
  • Begum MA; State Key Laboratory of Rice Biology; Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects; and Institute of Insect Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
  • Zhang CH; State Key Laboratory of Rice Biology; Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects; and Institute of Insect Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
  • Shi XX; State Key Laboratory of Rice Biology; Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects; and Institute of Insect Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
  • Jiao WJ; State Key Laboratory of Rice Biology; Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects; and Institute of Insect Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
  • Zhang YR; State Key Laboratory of Rice Biology; Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects; and Institute of Insect Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
  • Yuan JQ; State Key Laboratory of Rice Biology; Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects; and Institute of Insect Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
  • Li HY; Center for Chemical Analysis and Detection, Zhejiang University, Hangzhou, Zhejiang, China.
  • Yang Q; Institute of Biotechnology, Zhejiang University, Hangzhou, Zhejiang, China.
  • Mao C; Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, China.
  • Zhu ZR; Department of Medicine, State University of New York at Stony Brook. Stony Brook, NY, USA.
Insect Mol Biol ; 26(4): 392-402, 2017 08.
Article en En | MEDLINE | ID: mdl-28374513
The neutral sphingomyelinase (nSMase) 1 homologue gene LsSMase was cloned from Laodelphax striatellus, a direct sap-sucker and virus vector of gramineous plants, and expressed via a Bac to Bac baculovirus expression system. The LsSMase-enhanced green fluorescent protein fusion protein was located in the endoplasmic reticulum in a similar manner to mammalian nSMase 1. The biochemical properties of LsSMase were determined in detail. The optimal pH and temperature for recombinant LsSMase were 8 and 37 °C, respectively. LsSMase was an Mg2+ or Mn2+ dependent enzyme, but different concentration of each were needed. The activity of LsSMase was significantly stimulated by Ethylene glycol bis(2-aminoethyl ether)tetraacetic acid (EGTA), whereas it was inhibited by ethylenediaminetetraacetic acid. Millimolar concentrations of Zn2+ completely inhibited LsSMase. The reducing agents dithiothreitol and ß-mercaptoethanol varied in their effects on activity. Phospholipids were not found to stimulate LsSMase.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Esfingomielina Fosfodiesterasa / Hemípteros Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Insect Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2017 Tipo del documento: Article País de afiliación: China
Texto completo
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Esfingomielina Fosfodiesterasa / Hemípteros Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Insect Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2017 Tipo del documento: Article País de afiliación: China