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Comparison of Mitochondrial Function in Boar and Bull Spermatozoa Throughout Cryopreservation Based on JC-1 Staining.
Hu, C H; Zhuang, X J; Wei, Y M; Zhang, M; Lu, S S; Lu, Y Q; Yang, X G; Lu, K H.
Afiliación
  • Hu CH; College of Animal Science and Technology, Guangxi University, Nanning, China.
  • Zhuang XJ; College of Animal Science and Technology, Guangxi University, Nanning, China.
  • Wei YM; College of Animal Science and Technology, Guangxi University, Nanning, China.
  • Zhang M; College of Animal Science and Technology, Guangxi University, Nanning, China.
  • Lu SS; College of Animal Science and Technology, Guangxi University, Nanning, China.
  • Lu YQ; College of Animal Science and Technology, Guangxi University, Nanning, China.
  • Yang XG; College of Animal Science and Technology, Guangxi University, Nanning, China.
  • Lu KH; College of Animal Science and Technology, Guangxi University, Nanning, China. kehuanlu@163.com.
Cryo Letters ; 38(1): 75-79, 2017.
Article en En | MEDLINE | ID: mdl-28376143
ABSTRACT

BACKGROUND:

Poor reproductivity hampers the commercialization of cryopreserved boar semen.

OBJECTIVE:

This study was to determine the differences in the sperm mitochondrial function between boar and bull semen at different cryopreservation stages. MATERIALS AND

METHODS:

Boar and bull fresh, equilibrated, and frozen-thawed spermatozoa were evaluated for mitochondrial function using JC-1 under a fluorescent microscope.

RESULTS:

Bull and boar percentage of spermatozoa staining green (PSSG) showed no difference between fresh and equilibrated semen (P> 0.05). However, frozen-thawed bull and boar semen demonstrated significantly higher PSSG (P < 0.01) than fresh and equilibrated semen. Frozen-thawed boar semen represented a significantly higher PSSG (P < 0.01) than bull semen.

CONCLUSION:

Negative cryopreservation influence on boar and bull spermatozoa was not significantly produced by pre-freezing procedures, but rather by freezing and thawing. Cryopreservation has more pronounced negative effects on boar than on bull spermatozoa, which partly explains lagged commercialization of frozen boar semen.
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Bases de datos: MEDLINE Asunto principal: Preservación de Semen / Espermatozoides / Criopreservación / Mitocondrias Límite: Animals Idioma: En Revista: Cryo Letters Asunto de la revista: BIOLOGIA / QUIMICA Año: 2017 Tipo del documento: Article País de afiliación: China
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Bases de datos: MEDLINE Asunto principal: Preservación de Semen / Espermatozoides / Criopreservación / Mitocondrias Límite: Animals Idioma: En Revista: Cryo Letters Asunto de la revista: BIOLOGIA / QUIMICA Año: 2017 Tipo del documento: Article País de afiliación: China