Mesoporous Silica Nanoparticle-Coated Microneedle Arrays for Intradermal Antigen Delivery.
Pharm Res
; 34(8): 1693-1706, 2017 08.
Article
en En
| MEDLINE
| ID: mdl-28536970
ABSTRACT
PURPOSE:
To develop a new intradermal antigen delivery system by coating microneedle arrays with lipid bilayer-coated, antigen-loaded mesoporous silica nanoparticles (LB-MSN-OVA).METHODS:
Synthesis of MSNs with 10-nm pores was performed and the nanoparticles were loaded with the model antigen ovalbumin (OVA), and coated with a lipid bilayer (LB-MSN-OVA). The uptake of LB-MSN-OVA by bone marrow-derived dendritic cells (BDMCs) was studied by flow cytometry. The designed LB-MSN-OVA were coated onto pH-sensitive pyridine-modified microneedle arrays and the delivery of LB-MSN-OVA into ex vivo human skin was studied.RESULTS:
The synthesized MSNs demonstrated efficient loading of OVA with a maximum loading capacity of about 34% and the lipid bilayer enhanced the colloidal stability of the MSNs. Uptake of OVA loaded in LB-MSN-OVA by BMDCs was higher than that of free OVA, suggesting effective targeting of LB-MSN-OVA to antigen-presenting cells. Microneedles were readily coated with LB-MSN-OVA at pH 5.8, yielding 1.5 µg of encapsulated OVA per microneedle array. Finally, as a result of the pyridine modification, LB-MSN-OVA were effectively released from the microneedles upon piercing the skin.CONCLUSION:
Microneedle arrays coated with LB-MSN-OVA were successfully developed and shown to be suitable for intradermal delivery of the encapsulated protein antigen.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Ovalbúmina
/
Dióxido de Silicio
/
Nanopartículas
/
Antígenos
/
Agujas
Límite:
Humans
Idioma:
En
Revista:
Pharm Res
Año:
2017
Tipo del documento:
Article
País de afiliación:
Países Bajos