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Mesoporous Silica Nanoparticle-Coated Microneedle Arrays for Intradermal Antigen Delivery.
Tu, Jing; Du, Guangsheng; Reza Nejadnik, M; Mönkäre, Juha; van der Maaden, Koen; Bomans, Paul H H; Sommerdijk, Nico A J M; Slütter, Bram; Jiskoot, Wim; Bouwstra, Joke A; Kros, Alexander.
Afiliación
  • Tu J; Department of Supramolecular & Biomaterials Chemistry, Leiden Institute of Chemistry (LIC), Leiden University, Leiden, 2300, RA, The Netherlands.
  • Du G; Division of Drug Delivery Technology, Cluster BioTherapeutics, Leiden Academic Centre for Drug Research (LACDR), Leiden University, Leiden, 2300, RA, The Netherlands.
  • Reza Nejadnik M; Division of Drug Delivery Technology, Cluster BioTherapeutics, Leiden Academic Centre for Drug Research (LACDR), Leiden University, Leiden, 2300, RA, The Netherlands.
  • Mönkäre J; Division of Drug Delivery Technology, Cluster BioTherapeutics, Leiden Academic Centre for Drug Research (LACDR), Leiden University, Leiden, 2300, RA, The Netherlands.
  • van der Maaden K; Division of Drug Delivery Technology, Cluster BioTherapeutics, Leiden Academic Centre for Drug Research (LACDR), Leiden University, Leiden, 2300, RA, The Netherlands.
  • Bomans PHH; Laboratory of Materials and Interface Chemistry & Center of Multiscale Electron Microscopy, Department of Chemical Engineering and Chemistry, and Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, 5600, MB, The Netherlands.
  • Sommerdijk NAJM; Laboratory of Materials and Interface Chemistry & Center of Multiscale Electron Microscopy, Department of Chemical Engineering and Chemistry, and Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, 5600, MB, The Netherlands.
  • Slütter B; Division of Drug Delivery Technology, Cluster BioTherapeutics, Leiden Academic Centre for Drug Research (LACDR), Leiden University, Leiden, 2300, RA, The Netherlands.
  • Jiskoot W; Division of Biopharmaceutics, Cluster BioTherapeutics, Leiden Academic Centre for Drug Research (LACDR), Leiden University, Leiden, 2300, RA, The Netherlands.
  • Bouwstra JA; Division of Drug Delivery Technology, Cluster BioTherapeutics, Leiden Academic Centre for Drug Research (LACDR), Leiden University, Leiden, 2300, RA, The Netherlands.
  • Kros A; Division of Drug Delivery Technology, Cluster BioTherapeutics, Leiden Academic Centre for Drug Research (LACDR), Leiden University, Leiden, 2300, RA, The Netherlands. bouwstra@lacdr.leidenuniv.nl.
Pharm Res ; 34(8): 1693-1706, 2017 08.
Article en En | MEDLINE | ID: mdl-28536970
ABSTRACT

PURPOSE:

To develop a new intradermal antigen delivery system by coating microneedle arrays with lipid bilayer-coated, antigen-loaded mesoporous silica nanoparticles (LB-MSN-OVA).

METHODS:

Synthesis of MSNs with 10-nm pores was performed and the nanoparticles were loaded with the model antigen ovalbumin (OVA), and coated with a lipid bilayer (LB-MSN-OVA). The uptake of LB-MSN-OVA by bone marrow-derived dendritic cells (BDMCs) was studied by flow cytometry. The designed LB-MSN-OVA were coated onto pH-sensitive pyridine-modified microneedle arrays and the delivery of LB-MSN-OVA into ex vivo human skin was studied.

RESULTS:

The synthesized MSNs demonstrated efficient loading of OVA with a maximum loading capacity of about 34% and the lipid bilayer enhanced the colloidal stability of the MSNs. Uptake of OVA loaded in LB-MSN-OVA by BMDCs was higher than that of free OVA, suggesting effective targeting of LB-MSN-OVA to antigen-presenting cells. Microneedles were readily coated with LB-MSN-OVA at pH 5.8, yielding 1.5 µg of encapsulated OVA per microneedle array. Finally, as a result of the pyridine modification, LB-MSN-OVA were effectively released from the microneedles upon piercing the skin.

CONCLUSION:

Microneedle arrays coated with LB-MSN-OVA were successfully developed and shown to be suitable for intradermal delivery of the encapsulated protein antigen.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Ovalbúmina / Dióxido de Silicio / Nanopartículas / Antígenos / Agujas Límite: Humans Idioma: En Revista: Pharm Res Año: 2017 Tipo del documento: Article País de afiliación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Ovalbúmina / Dióxido de Silicio / Nanopartículas / Antígenos / Agujas Límite: Humans Idioma: En Revista: Pharm Res Año: 2017 Tipo del documento: Article País de afiliación: Países Bajos