Structure of the Arabidopsis TOPLESS corepressor provides insight into the evolution of transcriptional repression.
Proc Natl Acad Sci U S A
; 114(30): 8107-8112, 2017 07 25.
Article
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| MEDLINE
| ID: mdl-28698367
ABSTRACT
Transcriptional repression involves a class of proteins called corepressors that link transcription factors to chromatin remodeling complexes. In plants such as Arabidopsis thaliana, the most prominent corepressor is TOPLESS (TPL), which plays a key role in hormone signaling and development. Here we present the crystallographic structure of the Arabidopsis TPL N-terminal region comprising the LisH and CTLH (C-terminal to LisH) domains and a newly identified third region, which corresponds to a CRA domain. Comparing the structure of TPL with the mammalian TBL1, which shares a similar domain structure and performs a parallel corepressor function, revealed that the plant TPLs have evolved a new tetramerization interface and unique and highly conserved surface for interaction with repressors. Using site-directed mutagenesis, we validated those surfaces in vitro and in vivo and showed that TPL tetramerization and repressor binding are interdependent. Our results illustrate how evolution used a common set of protein domains to create a diversity of corepressors, achieving similar properties with different molecular solutions.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Arabidopsis
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Regulación de la Expresión Génica de las Plantas
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Evolución Molecular
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Proteínas de Arabidopsis
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Proteínas Co-Represoras
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Año:
2017
Tipo del documento:
Article
País de afiliación:
Francia