Genetic engineering as a tool for the generation of mouse models to understand disease phenotypes and gene function.
Curr Opin Biotechnol
; 48: 228-233, 2017 12.
Article
en En
| MEDLINE
| ID: mdl-28704794
ABSTRACT
The usage of mouse models has been vital for biomedical research over the last decades, yet the generation of these models has been extremely difficult and labor-intensive. The identification and generation of nucleases able to introduce site-specific DNA double-strand breaks, particularly the CRIPSR/Cas system, is a major breakthrough for this field as the endogenous DNA repair machinery can be hijacked to specifically introduce genome modifications at these sites. This allows for the time-efficient and cost-efficient generation of mouse models by delivery of designer nucleases together with donor DNA into fertilized oocytes.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Ingeniería Genética
/
Modelos Animales de Enfermedad
/
Genes
Límite:
Animals
Idioma:
En
Revista:
Curr Opin Biotechnol
Asunto de la revista:
BIOTECNOLOGIA
Año:
2017
Tipo del documento:
Article
País de afiliación:
Alemania