Evaluation of a PCR assay on overgrown individual fecal samples cultured for Mycobacterium avium subsp. paratuberculosis.
J Vet Diagn Invest
; 29(6): 912-915, 2017 Nov.
Article
en En
| MEDLINE
| ID: mdl-28782422
ABSTRACT
Microbial overgrowth can interfere with Mycobacterium avium subsp. paratuberculosis (MAP) growth and detection. We estimated the percentage of positive samples by PCR performed on the incubated media of individual fecal samples classified as non-interpretable (NI) by bacteriologic culture of liquid media. A total of 262 liquid cultures declared NI and 88 samples declared negative were included in the study. MAP DNA was detected in 7 NI samples (2.7%; 95% CI 1.1-5.4%) and in 1 negative sample (1.1%; 95% CI 0.3-6.2%). The PCR allowed the detection of MAP-positive samples that had been missed in the initial bacteriologic culture. However, the benefit of these few additional positive results must be weighed against the additional costs incurred. Using PCR to classify overgrown cultures optimizes the detection process and eliminates the NI outcome.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Paratuberculosis
/
Enfermedades de los Bovinos
/
Mycobacterium avium subsp. paratuberculosis
/
Reacción en Cadena en Tiempo Real de la Polimerasa
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Vet Diagn Invest
Asunto de la revista:
MEDICINA VETERINARIA
Año:
2017
Tipo del documento:
Article