Cell metabolomics reveals the neurotoxicity mechanism of cadmium in PC12 cells.
Ecotoxicol Environ Saf
; 147: 26-33, 2018 Jan.
Article
en En
| MEDLINE
| ID: mdl-28822947
ABSTRACT
The heavy metals such as cadmium (Cd) can induce neurotoxicity. Extensive studies about the effects of Cd on human health have been reported, however, a systematic investigation on the molecular mechanisms of the effects of Cd on central nervous system is still needed. In this paper, the neuronal PC-12 cells were treated with a series of concentrations of CdCl2 for 48h. Then the cytotoxicity was evaluated by MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. The IC15 value (15% inhibiting concentration) was selected for further mechanism studies. After PC-12 cells incubated with CdCl2 at a dose of IC15 for 48h, the intracellular and extracellular metabolites were profiled using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS)-based cell metabolomics approach. As found, the effects of the heavy metal Cd produced on the PC-12 cell viability were dose-dependent. The metabolic changes were involved in the glycolysis and gluconeogenesis, biopterin metabolism, tryptophan metabolism, tyrosine metabolism, glycerophospholipid metabolism, and fatty acids beta-oxidation. These could cause the perturbation of cell membrane, redox balance, energy supply, cellular detoxification, further affecting the cellular proliferation and apoptosis and other cellular activities.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Cadmio
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Síndromes de Neurotoxicidad
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Contaminantes Ambientales
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Metaboloma
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Metabolómica
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Neuronas
Límite:
Animals
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Humans
Idioma:
En
Revista:
Ecotoxicol Environ Saf
Año:
2018
Tipo del documento:
Article
País de afiliación:
China