An Effective Cell Coculture Platform Based on the Electrospun Microtube Array Membrane for Nerve Regeneration.
Cells Tissues Organs
; 204(3-4): 179-190, 2017.
Article
en En
| MEDLINE
| ID: mdl-28848167
ABSTRACT
Recently, a novel substrate known as an electrospun polylactic acid (PLLA) microtube array membrane (MTAM) was successfully developed as a cell coculture platform. Structurally, this substrate is made up of one-to-one connected, ultrathin, submicron scale fibers that are arranged in an arrayed formation. Its unique structure confers several key advantages which are beneficial in a cell coculture system. In this study, the interaction between rat fetal neural stem cells (NSC) and astrocytes was examined by comparing the outcome of a typical Transwell-based coculture system and that of an electrospun PLLA MTAM-based coculture system. Compared to tissue culture polystyrene (TCP) and Transwell coculture inserts, a superior cell viability of NSC was observed when cultured in lumens of electrospun PLLA MTAM (with supportive immunostaining images). Reverse transcription polymerase chain reaction revealed a strong interaction between astrocytes and NSC through a higher expression of doublecortin and a lower expression of nestin. These data demonstrate that MTAM is clearly a better coculture platform than the traditional Transwell system.
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Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Astrocitos
/
Ingeniería de Tejidos
/
Regeneración Nerviosa
Límite:
Animals
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Humans
Idioma:
En
Revista:
Cells Tissues Organs
Asunto de la revista:
ANATOMIA
Año:
2017
Tipo del documento:
Article