[Isolation and identification of berberine from endophytic fungi HL-Y-3].
Zhongguo Zhong Yao Za Zhi
; 41(16): 2998-3001, 2016 Aug.
Article
en Zh
| MEDLINE
| ID: mdl-28920338
ABSTRACT
The endophytic fungi HL-Y-3, which was isolated from the healthy leaves of Coptis chinensis, produced berberine when grown in the PDA culture medium. The presence of berberine was confirmed by the chromatographic and spectroscopic analyses. The yield of berberine was recorded as 9.313 µgâ¢g⻹ by HPLC. The strain HL-Y-3 was identified as Alternaria sp.by morphological observation and 5.8S rDNA-ITS sequence analysis.The separation and purification of constituents were performed by PTLC. The mass spectrometry (MS) of the analyte was shown to be identical with authentic berberine.Further analysis with nuclear magnetic resonance (NMR) spectroscopy to showed that the chemical structure of the fungal berberine was identical with authentic berberine. The research provided new resources for the utilization of berberine.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Berberina
/
Coptis
/
Alternaria
Tipo de estudio:
Diagnostic_studies
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Asunto de la revista:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Año:
2016
Tipo del documento:
Article
País de afiliación:
China