[Relaxation effect of buddleoside combined with luteolin on isolated vessels in vivo and its mechanism].
Zhongguo Zhong Yao Za Zhi
; 42(7): 1370-1375, 2017 Apr.
Article
en Zh
| MEDLINE
| ID: mdl-29052401
ABSTRACT
To study the relaxation effect of buddleoside combined with luteolin on aortic rings in SD rats and its mechanism. The effect of buddleoside alone(7.5×10â»6gâ¢mL⻹), luteolin alone(7.5×10â»6gâ¢mL⻹) and the combination of buddleoside and luteolin(1â¶4) on norepinephrine-induced contractility of complete, endothelium-denuded, and L-NAME and indomethacin-pretreated thoracic aorta in SD rats were observed in the in vitro ring tension test. Western blot was used to detect p-Akt and p-eNOS protein expressions in the thoracic aorta. The experimental results showed that buddleoside combined with luteolin could significantly increase the relaxation rate of blood vessels and endothelium and L-NAME-pretreated vascular rings compared with the two single administrations. And buddleoside combined with luteolin could also significantly increase p-Akt and p-eNOS protein expressions.The results suggested that the combination of buddleoside and luteolin could effectively relax the blood vessel, and the mechanism may be to increase the synthesis and release of NO and reach the role of relaxing blood vessel by activating PI3K/Akt/NO signaling pathway and enhancing the activity of eNOS.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Aorta Torácica
/
Vasodilatación
/
Luteolina
/
Glicósidos
Límite:
Animals
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Asunto de la revista:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Año:
2017
Tipo del documento:
Article
País de afiliación:
China