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Correlations in Scattered X-Ray Laser Pulses Reveal Nanoscale Structural Features of Viruses.
Kurta, Ruslan P; Donatelli, Jeffrey J; Yoon, Chun Hong; Berntsen, Peter; Bielecki, Johan; Daurer, Benedikt J; DeMirci, Hasan; Fromme, Petra; Hantke, Max Felix; Maia, Filipe R N C; Munke, Anna; Nettelblad, Carl; Pande, Kanupriya; Reddy, Hemanth K N; Sellberg, Jonas A; Sierra, Raymond G; Svenda, Martin; van der Schot, Gijs; Vartanyants, Ivan A; Williams, Garth J; Xavier, P Lourdu; Aquila, Andrew; Zwart, Peter H; Mancuso, Adrian P.
Afiliación
  • Kurta RP; European XFEL GmbH, Holzkoppel 4, D-22869 Schenefeld, Germany.
  • Donatelli JJ; Mathematics Department, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, California 94720, USA.
  • Yoon CH; Center for Advanced Mathematics for Energy Research Applications, 1 Cyclotron Road, Berkeley, California 94720, USA.
  • Berntsen P; Linac Coherent Light Source, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
  • Bielecki J; Australian Research Council Centre of Excellence in Advanced Molecular Imaging, La Trobe Institute for Molecular Science, La Trobe University, Melbourne 3086, Australia.
  • Daurer BJ; European XFEL GmbH, Holzkoppel 4, D-22869 Schenefeld, Germany.
  • DeMirci H; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, SE-751 24 Uppsala, Sweden.
  • Fromme P; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, SE-751 24 Uppsala, Sweden.
  • Hantke MF; Biosciences Division, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
  • Maia FRNC; Stanford PULSE Institute, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
  • Munke A; Biodesign Center for Applied Structural Discovery and School of Molecular Sciences, Arizona State University, Tempe, Arizona 85287-1604, USA.
  • Nettelblad C; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, SE-751 24 Uppsala, Sweden.
  • Pande K; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, SE-751 24 Uppsala, Sweden.
  • Reddy HKN; NERSC, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
  • Sellberg JA; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, SE-751 24 Uppsala, Sweden.
  • Sierra RG; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, SE-751 24 Uppsala, Sweden.
  • Svenda M; Division of Scientific Computing, Science for Life Laboratory, Department of Information Technology, Uppsala University, SE-751 05 Uppsala, Sweden.
  • van der Schot G; Center for Advanced Mathematics for Energy Research Applications, 1 Cyclotron Road, Berkeley, California 94720, USA.
  • Vartanyants IA; Molecular Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, California 94720, USA.
  • Williams GJ; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, SE-751 24 Uppsala, Sweden.
  • Xavier PL; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, SE-751 24 Uppsala, Sweden.
  • Aquila A; Biomedical and X-Ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, Stockholm SE-106 91, Sweden.
  • Zwart PH; Linac Coherent Light Source, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
  • Mancuso AP; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, SE-751 24 Uppsala, Sweden.
Phys Rev Lett ; 119(15): 158102, 2017 Oct 13.
Article en En | MEDLINE | ID: mdl-29077445
ABSTRACT
We use extremely bright and ultrashort pulses from an x-ray free-electron laser (XFEL) to measure correlations in x rays scattered from individual bioparticles. This allows us to go beyond the traditional crystallography and single-particle imaging approaches for structure investigations. We employ angular correlations to recover the three-dimensional (3D) structure of nanoscale viruses from x-ray diffraction data measured at the Linac Coherent Light Source. Correlations provide us with a comprehensive structural fingerprint of a 3D virus, which we use both for model-based and ab initio structure recovery. The analyses reveal a clear indication that the structure of the viruses deviates from the expected perfect icosahedral symmetry. Our results anticipate exciting opportunities for XFEL studies of the structure and dynamics of nanoscale objects by means of angular correlations.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Virus / Difracción de Rayos X Tipo de estudio: Diagnostic_studies Idioma: En Revista: Phys Rev Lett Año: 2017 Tipo del documento: Article País de afiliación: Alemania
Texto completo
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PubMed Links
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Virus / Difracción de Rayos X Tipo de estudio: Diagnostic_studies Idioma: En Revista: Phys Rev Lett Año: 2017 Tipo del documento: Article País de afiliación: Alemania