The Secretome of Microglia Regulate Neural Stem Cell Function.
Neuroscience
; 405: 92-102, 2019 05 01.
Article
en En
| MEDLINE
| ID: mdl-29101080
ABSTRACT
Brain injury is associated with neuroinflammation, and microglia are key players in this process. Microglia can acquire pro-inflammatory or anti-inflammatory properties, but how this affects neural stem cells (NSCs) remains controversial. Here, NSCs were grown in conditioned media collected from either non-stimulated microglia, or microglia stimulated with pro- or anti-inflammatory agents. NSC survival, proliferation, migration, and differentiation were investigated thereafter. We found that NSCs kept in conditioned medium from the anti-inflammatory microglial subtype had better survival, increased migration, and lower astrocytic differentiation compared to NSCs grown in conditioned medium collected from the pro-inflammatory subtype. Finally, we found that NSCs differentiated in microglial conditioned media generated cells expressing the pro-inflammatory chemokine CCL2, most pronounced when differentiated in medium from the pro-inflammatory microglia subtype. Our results show that microglial subtypes regulate NSCs differently and induce generation of cells with inflammatory properties.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Citocinas
/
Microglía
/
Células-Madre Neurales
Límite:
Animals
Idioma:
En
Revista:
Neuroscience
Año:
2019
Tipo del documento:
Article
País de afiliación:
Suecia