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Antibody test for Legionella pneumophila detection.
Párraga-Niño, Noemí; Quero, Sara; Uria, Naroa; Castillo-Fernandez, Oscar; Jimenez-Ezenarro, Josune; Muñoz, Francesc-Xavier; Sabrià, Miquel; Garcia-Nuñez, Marian.
Afiliación
  • Párraga-Niño N; Unitat de Malalties Infeccioses, Fundació Institut d'Investigació Germans Trias I Pujol, Badalona, Spain E-08916; CIBER de Enfermedades Respiratorias, Madrid, Spain E-28029.
  • Quero S; Unitat de Malalties Infeccioses, Fundació Institut d'Investigació Germans Trias I Pujol, Badalona, Spain E-08916.
  • Uria N; Institute of Microelectronics of Barcelona IMB-CNM (CSIC), Cerdanyola del Vallès, Spain E-08193.
  • Castillo-Fernandez O; Institute of Microelectronics of Barcelona IMB-CNM (CSIC), Cerdanyola del Vallès, Spain E-08193.
  • Jimenez-Ezenarro J; Institute of Microelectronics of Barcelona IMB-CNM (CSIC), Cerdanyola del Vallès, Spain E-08193.
  • Muñoz FX; Institute of Microelectronics of Barcelona IMB-CNM (CSIC), Cerdanyola del Vallès, Spain E-08193.
  • Sabrià M; Unitat de Malalties Infeccioses, Fundació Institut d'Investigació Germans Trias I Pujol, Badalona, Spain E-08916; CIBER de Enfermedades Respiratorias, Madrid, Spain E-28029; Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain E-08193. Electronic address: msabria.germanstrias@gencat.cat.
  • Garcia-Nuñez M; Unitat de Malalties Infeccioses, Fundació Institut d'Investigació Germans Trias I Pujol, Badalona, Spain E-08916; CIBER de Enfermedades Respiratorias, Madrid, Spain E-28029.
Diagn Microbiol Infect Dis ; 90(2): 85-89, 2018 Feb.
Article en En | MEDLINE | ID: mdl-29217420
Legionella pneumophila is responsible for Legionnaires' disease (LD). Its detection in both environmental and clinical samples is mainly performed by culture plate method which requires up to 10days to obtain results. Nowadays, there are commercial antibodies against this bacterium, but they have not been tested against all subgroups of L. pneumophila sg 1 or serogroups 1-16 or their cross-reactions with other non-Legionella bacteria. Indeed, many of these antibodies became available when only 8 serogroups of L. pneumophila had been described. We tested 7 antibodies and found that 2 (Mab 8/5 and OBT) specifically detected all the subgroups of L. pneumophila sg 1, one without cross-reactions (Mab8/5). Moreover, the LP3IIG2 antibody detected almost all serogroups tested with lower rates of cross-reactivity, resulting in a specific sensitive antibody for the detection of L. pneumophila. LP3IIG2 presented higher rate of cross-reactivity against respiratory non-Legionella isolates, thereby contraindicating its clinical applicability.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Enfermedad de los Legionarios / Serotipificación / Técnicas de Tipificación Bacteriana / Legionella pneumophila / Anticuerpos Antibacterianos Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Diagn Microbiol Infect Dis Año: 2018 Tipo del documento: Article

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Enfermedad de los Legionarios / Serotipificación / Técnicas de Tipificación Bacteriana / Legionella pneumophila / Anticuerpos Antibacterianos Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Diagn Microbiol Infect Dis Año: 2018 Tipo del documento: Article