Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice.
J Vis Exp
; (131)2018 01 06.
Article
en En
| MEDLINE
| ID: mdl-29364278
ABSTRACT
Bone marrow stromal cells (BMSCs) constitute a cell population routinely used as a representation of mesenchymal stem cells in vitro. They reside within the bone marrow cavity alongside hematopoietic stem cells (HSCs), which can give rise to red blood cells, immune progenitors, and osteoclasts. Thus, extractions of cell populations from the bone marrow results in a very heterogeneous mix of various cell populations, which can present challenges in experimental design and confound data interpretation. Several isolation and culture techniques have been developed in laboratories in order to obtain more or less homogeneous populations of BMSCs and HSCs invitro. Here, we present two methods for isolation of BMSCs and HSCs from mouse long bones one method that yields a mixed population of BMSCs and HSCs and one method that attempts to separate the two cell populations based on adherence. Both methods provide cells suitable for osteogenic and adipogenic differentiation experiments as well as functional assays.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Osteoclastos
/
Células de la Médula Ósea
/
Células Madre Mesenquimatosas
Límite:
Animals
Idioma:
En
Revista:
J Vis Exp
Año:
2018
Tipo del documento:
Article