ES cell-derived presomitic mesoderm-like tissues for analysis of synchronized oscillations in the segmentation clock.
Development
; 145(4)2018 02 14.
Article
en En
| MEDLINE
| ID: mdl-29437832
ABSTRACT
Somites are periodically formed by segmentation of the anterior parts of the presomitic mesoderm (PSM). In the mouse embryo, this periodicity is controlled by the segmentation clock gene Hes7, which exhibits wave-like oscillatory expression in the PSM. Despite intensive studies, the exact mechanism of such synchronous oscillatory dynamics of Hes7 expression still remains to be analyzed. Detailed analysis of the segmentation clock has been hampered because it requires the use of live embryos, and establishment of an in vitro culture system would facilitate such analyses. Here, we established a simple and efficient method to generate mouse ES cell-derived PSM-like tissues, in which Hes7 expression oscillates like traveling waves. In these tissues, Hes7 oscillation is synchronized between neighboring cells, and the posterior-anterior axis is self-organized as the central-peripheral axis. This method is applicable to chemical-library screening and will facilitate the analysis of the molecular nature of the segmentation clock.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Somitos
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Tipificación del Cuerpo
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Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico
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Células Madre Embrionarias
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Mesodermo
Límite:
Animals
Idioma:
En
Revista:
Development
Asunto de la revista:
BIOLOGIA
/
EMBRIOLOGIA
Año:
2018
Tipo del documento:
Article
País de afiliación:
Japón