The Biology of CRISPR-Cas: Backward and Forward.
Cell
; 172(6): 1239-1259, 2018 03 08.
Article
en En
| MEDLINE
| ID: mdl-29522745
ABSTRACT
In bacteria and archaea, clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system against phages and other foreign genetic elements. Here, we review the biology of the diverse CRISPR-Cas systems and the major progress achieved in recent years in understanding the underlying mechanisms of the three stages of CRISPR-Cas immunity adaptation, crRNA biogenesis, and interference. The ecology and regulation of CRISPR-Cas in the context of phage infection, the roles of these systems beyond immunity, and the open questions that propel the field forward are also discussed.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Bacterias
/
Bacteriófagos
/
Biología
/
Sistemas CRISPR-Cas
Idioma:
En
Revista:
Cell
Año:
2018
Tipo del documento:
Article
País de afiliación:
Alemania