Deuteration of human carbonic anhydrase for neutron crystallography: Cell culture media, protein thermostability, and crystallization behavior.

Koruza, K; Lafumat, B; Végvári, Á; Knecht, W; Fisher, S Z

Koruza, K; Lafumat, B; Végvári, Á; Knecht, W; Fisher, S Z.

Afiliación

Koruza K; Department of Biology & Lund Protein Production Platform, Lund University, Sölvegatan 35, Lund 22362, Sweden.
Lafumat B; Department of Biology & Lund Protein Production Platform, Lund University, Sölvegatan 35, Lund 22362, Sweden.
Végvári Á; Department of Medical Biochemistry & Biophysics, Karolinska Institute, Scheeles väg 2, Stockholm 17177, Sweden.
Knecht W; Department of Biology & Lund Protein Production Platform, Lund University, Sölvegatan 35, Lund 22362, Sweden.
Fisher SZ; Department of Biology & Lund Protein Production Platform, Lund University, Sölvegatan 35, Lund 22362, Sweden; Scientific Activities Division, European Spallation Source ERIC, Tunavägen 24, Lund 22100, Sweden. Electronic address: zoe.fisher@esss.se.

Arch Biochem Biophys ; 645: 26-33, 2018 05 01.

Article en En | MEDLINE | ID: mdl-29524429

Asunto(s)

Anhidrasas Carbónicas/química; Medios de Cultivo/química; Deuterio/química; Neutrones; Temperatura; Cristalización; Cristalografía; Estabilidad de Enzimas; Humanos; Desplegamiento Proteico

Palabras clave

Carbonic anhydrase; Deuteration; Minimal media; Neutron scattering; Thermal stability

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Temperatura / Anhidrasas Carbónicas / Medios de Cultivo / Deuterio / Neutrones Límite: Humans Idioma: En Revista: Arch Biochem Biophys Año: 2018 Tipo del documento: Article País de afiliación: Suecia

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