[Cloning and expression analysis of squalene synthase gene in Tripterygium wilfordii].

ABSTRACT

In this paper, we cloned the full-length cDNA of TwSQS from Tripterygium wilfordii suspension cells(Gen Bank KR401220) and performed the bioinformation and m RNA expression analysis. The expression after methyl jasmonate(MJ) treatment of the gene was detected by RT-PCR. The full-length cDNA of TwSQS was 1 800 bp containing a 1 242 bp open reading frame(ORF) encoding a polypeptide of 413 amino acids. The theoretical isoelectric point(p I) was 7.94 and the calculate molecular weight was about 47.20 k D. The relative expression level of TwSQS was deduced by MJ and reached the highest at 4 h after the treatment. The gene information we got in this study enriched the biosynthesis pathway of triterpenoids in Tripterygium wilfordii and laid foundation for further studies.