A33 antibody-functionalized exosomes for targeted delivery of doxorubicin against colorectal cancer.
Nanomedicine
; 14(7): 1973-1985, 2018 10.
Article
en En
| MEDLINE
| ID: mdl-29935333
ABSTRACT
Exosomes have emerged as a promising drug carrier with low immunogenicity, high biocompatibility and delivery efficiency. Here in, we isolated exosomes from A33-positive LIM1215 cells (A33-Exo) and loaded them with doxorubicin (Dox). Furthermore, we coated surface-carboxyl superparamagnetic iron oxide nanoparticles (US) with A33 antibodies (A33Ab-US), expecting that these A33 antibodies on the surface of the nanoparticles could bind to A33-positive exosomes and form a complex (A33Ab-US-Exo/Dox) to target A33-positive colon cancer cells. The results showed that A33Ab-US-Exo/Dox had good binding affinity and antiproliferative effect in LIM1215 cells, as shown by increased uptake of the complex. In vivo study showed that A33Ab-US-Exo/Dox had an excellent tumor targeting ability, and was able to inhibit tumor growth and prolong the survival of the mice with reduced cardiotoxicity. In summary, exosomes functionalized by targeting ligands through coating with high-density antibodies may prove to be a novel delivery system for targeted drugs against human cancers.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Glicoproteínas de Membrana
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Neoplasias Colorrectales
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Doxorrubicina
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Sistemas de Liberación de Medicamentos
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Exosomas
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Anticuerpos Monoclonales
Límite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Nanomedicine
Asunto de la revista:
BIOTECNOLOGIA
Año:
2018
Tipo del documento:
Article
País de afiliación:
China