Rationally Engineered Tandem Facial Amphiphiles for Improved Membrane Protein Stabilization Efficacy.
Chembiochem
; 19(20): 2225-2232, 2018 10 18.
Article
en En
| MEDLINE
| ID: mdl-30070754
ABSTRACT
A new family of tandem facial glucosides/maltosides (TFGs/TFMs) for membrane protein manipulation was prepared. The best detergent varied depending on the hydrophobic thickness of the target protein, but ether-based TFMs (TFM-C0E, TFM-C3E, and TFM-C5E) were notable for their ability to confer higher membrane protein stability than the previously developed amide-based TFA-1 (P.â
S. Chae, K. Gotfryd, J. Pacyna, L.â
J.â
W. Miercke, S.â
G.â
F. Rasmussen, R.â
A. Robbins, R.â
R. Rana, C.â
J. Loland, B. Kobilka, R. Stroud, B. Byrne, U. Gether, S.â
H. Gellman, J. Am. Chem. Soc. 2010, 132, 16750-16752). Thus, this study not only introduces novel agents with the potential to be used in membrane protein research but also highlights the importance of both the hydrophobic length and linker functionality of the detergent in stabilizing membrane proteins.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Receptores Adrenérgicos beta 2
/
Sistemas de Transporte de Aminoácidos
/
Simportadores
/
Detergentes
/
Proteínas de la Membrana
Límite:
Humans
Idioma:
En
Revista:
Chembiochem
Asunto de la revista:
BIOQUIMICA
Año:
2018
Tipo del documento:
Article