A copper transcription factor, AfMac1, regulates both iron and copper homeostasis in the opportunistic fungal pathogen Aspergillus fumigatus.
Biochem J
; 475(17): 2831-2845, 2018 09 11.
Article
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| MEDLINE
| ID: mdl-30072493
ABSTRACT
Although iron and copper are co-ordinately regulated in living cells, the homeostatic effects of each of these metals on the other remain unknown. Here, we show the function of AfMac1, a transcriptional activator of the copper and iron regulons of Aspergillus fumigatus, on the interaction between iron and copper. In addition to the copper-specific AfMac1-binding motif 5'-TGTGCTCA-3' found in the promoter region of ctrC, the iron-specific AfMac1-binding motif 5'-AT(C/G)NN(A/T)T(A/C)-3' was identified in the iron regulon but not in the copper regulon by ChIP sequence analysis. Furthermore, mutation of the AfMac1-binding motif of sit1 eliminated AfMac1-mediated sit1 up-regulation. Interestingly, the regulation of gene expression in the iron regulon by AfMac1 was not affected by copper and vice versa AfMac1 localized to the nucleus under iron- or copper-depleted conditions, and AfMac1 was mostly detected in the cytoplasm under iron- or copper-replete conditions. Taken together, these results suggest that A. fumigatus independently regulates iron and copper homeostasis in a manner that involves AfMac1 and mutual interactions.
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1
Bases de datos:
MEDLINE
Asunto principal:
Aspergillus fumigatus
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Factores de Transcripción
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Proteínas Fúngicas
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Regulación Fúngica de la Expresión Génica
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Cobre
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Elementos de Respuesta
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Biochem J
Año:
2018
Tipo del documento:
Article