A multi-omic atlas of the human frontal cortex for aging and Alzheimer's disease research.
Sci Data
; 5: 180142, 2018 08 07.
Article
en En
| MEDLINE
| ID: mdl-30084846
ABSTRACT
We initiated the systematic profiling of the dorsolateral prefrontal cortex obtained from a subset of autopsied individuals enrolled in the Religious Orders Study (ROS) or the Rush Memory and Aging Project (MAP), which are jointly designed prospective studies of aging and dementia with detailed, longitudinal cognitive phenotyping during life and a quantitative, structured neuropathologic examination after death. They include over 3,322 subjects. Here, we outline the first generation of data including genome-wide genotypes (n=2,090), whole genome sequencing (n=1,179), DNA methylation (n=740), chromatin immunoprecipitation with sequencing using an anti-Histone 3 Lysine 9 acetylation (H3K9Ac) antibody (n=712), RNA sequencing (n=638), and miRNA profile (n=702). Generation of other omic data including ATACseq, proteomic and metabolomics profiles is ongoing. Thanks to its prospective design and recruitment of older, non-demented individuals, these data can be repurposed to investigate a large number of syndromic and quantitative neuroscience phenotypes. The many subjects that are cognitively non-impaired at death also offer insights into the biology of the human brain in older non-impaired individuals.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Genoma Humano
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Proteómica
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Enfermedad de Alzheimer
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Lóbulo Frontal
Tipo de estudio:
Observational_studies
Límite:
Aged
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Aged80
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Female
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Humans
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Male
Idioma:
En
Revista:
Sci Data
Año:
2018
Tipo del documento:
Article
País de afiliación:
Estados Unidos