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Inoculum effect of methicillin-susceptible Staphylococcus aureus against broad-spectrum beta-lactam antibiotics.
Song, Kyoung-Ho; Jung, Sook-In; Lee, Shinwon; Park, Sohee; Kim, Eu Suk; Park, Kyung-Hwa; Park, Wan Beom; Choe, Pyoeng Gyun; Kim, Young Keun; Kwak, Yee Gyung; Kim, Yeon-Sook; Jang, Hee-Chang; Kiem, Sungmin; Kim, Hye-In; Kim, Hong Bin.
Afiliación
  • Song KH; Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Republic of Korea.
  • Jung SI; Department of Infectious Diseases, Chonnam National University Medical School, Gwangju, Republic of Korea.
  • Lee S; Department of Internal Medicine, Pusan National University School of Medicine and Medical Research Institute, Pusan National University Hospital, 179 Gudeok-ro, Seo-gu, Busan, 49241, Republic of Korea. ebenezere.lee@gmail.com.
  • Park S; Department of Internal Medicine, Pusan National University School of Medicine and Medical Research Institute, Pusan National University Hospital, 179 Gudeok-ro, Seo-gu, Busan, 49241, Republic of Korea.
  • Kim ES; Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Republic of Korea.
  • Park KH; Department of Infectious Diseases, Chonnam National University Medical School, Gwangju, Republic of Korea.
  • Park WB; Department of Internal Medicine, Seoul National University Hospital, Seoul, Republic of Korea.
  • Choe PG; Department of Internal Medicine, Seoul National University Hospital, Seoul, Republic of Korea.
  • Kim YK; Department of Internal Medicine, Yonsei University Medical School, Wonju, Republic of Korea.
  • Kwak YG; Department of Internal Medicine, Inje University Ilsan Paik Hospital, Goyang, Republic of Korea.
  • Kim YS; Division of Infectious Diseases, Chungnam National University Hospital, Chungnam National University School of Medicine, Daejeon, Republic of Korea.
  • Jang HC; Department of Internal Medicine, Chonnam National University Hwasun Hospital, Hwasun, Jeollanam-do, Republic of Korea.
  • Kiem S; Department of Internal Medicine, Inje University College of Medicine, Busan, Republic of Korea.
  • Kim HI; Department of Internal Medicine, Daegu Fatima Hospital, Daegu, Republic of Korea.
  • Kim HB; Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Republic of Korea.
Eur J Clin Microbiol Infect Dis ; 38(1): 67-74, 2019 Jan.
Article en En | MEDLINE | ID: mdl-30269181
Scarce information concerning the inoculum effect (InE) of methicillin-susceptible Staphylococcus aureus (MSSA) against broad-spectrum ß-lactam antibiotics is available. We investigated the InE of MSSA against ceftriaxone, cefepime, meropenem, ampicillin/sulbactam and piperacillin/tazobactam. The bacteraemic MSSA isolates were collected at ten Korean general hospitals from Sep 2013 to Mar 2015. The InE was defined if MICs of antibiotics at high inoculum (HI, ~5 × 107 CFU/ml) increased beyond the susceptible range compared to those at standard inoculum (SI, ~5 × 105 CFU/ml). All isolates were sequenced for blaZ gene typing. Among 302 MSSA isolates, 254 (84.1%) were positive for blaZ; types A, B, C and D were 13.6%, 26.8%, 43.4% and 0.3%, respectively. Mean HI MICs of all tested antibiotics were significantly increased and increases in HI MIC of piperacillin/tazobactam (HI, 48.14 ± 4.08 vs. SI, 2.04 ± 0.08 mg/L, p < 0.001) and ampicillin/sulbactam (HI, 24.15 ± 1.27 vs. SI, 2.79 ± 0.11 mg/L, p < 0.001) were most prominent. No MSSA isolates exhibited meropenem InE, and few isolates exhibited cefepime (0.3%) and ceftriaxone (2.3%) InE, whereas 43.0% and 65.9% of MSSA isolates exhibited piperacillin/tazobactam and ampicillin/sulbactam InE, respectively. About 93% of type C blaZ versus 45% of non-type C exhibited ampicillin/sulbactam InE (p < 0.001) and 88% of type C blaZ versus 9% of non-type C exhibited piperacillin/tazobactam InE (p < 0.001). A large proportion of MSSA clinical isolates, especially those positive for type C blaZ, showed marked ampicillin/sulbactam InE and piperacillin/tazobactam.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Infecciones Estafilocócicas / Staphylococcus aureus / Beta-Lactamas / Antibacterianos Límite: Humans País/Región como asunto: Asia Idioma: En Revista: Eur J Clin Microbiol Infect Dis Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2019 Tipo del documento: Article

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Infecciones Estafilocócicas / Staphylococcus aureus / Beta-Lactamas / Antibacterianos Límite: Humans País/Región como asunto: Asia Idioma: En Revista: Eur J Clin Microbiol Infect Dis Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2019 Tipo del documento: Article