Adherens junction-associated pores mediate the intercellular transport of endosomes and cytoplasmic proteins.
Biochem Biophys Res Commun
; 505(3): 664-670, 2018 11 02.
Article
en En
| MEDLINE
| ID: mdl-30286955
ABSTRACT
Intercellular endosomes (IEs) are endocytosed vesicles shuttled through the adherens junctions (AJs) between two neighboring epidermal cells during Drosophila dorsal closure. The cell-to-cell transport of IEs requires DE-cadherin (DE-cad), microtubules (MTs) and kinesin. However, the mechanisms by which IEs can be transported through the AJs are unknown. Here, we demonstrate the presence of AJ-associated pores with MTs traversing through the pores. Live imaging allows direct visualization of IEs being transported through the AJ-associated pores. By using an optogenetic dimerization system, we observe that the dimerized IE-kinesin complexes move across AJs into the neighboring cell. The AJ-associated pores also allow intercellular movement of soluble proteins. Importantly, most epidermal cells form dorsoventral-oriented two-cell syncytia. Together, we present a model in which an AJ-associated pore mediates the intercellular transport of IEs and proteins between two cells in direct contact.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Endosomas
/
Uniones Adherentes
/
Citoplasma
/
Proteínas de Drosophila
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2018
Tipo del documento:
Article
País de afiliación:
Taiwán