Exposure to aflatoxin B1 interferes with locomotion and neural development in zebrafish embryos and larvae.
Chemosphere
; 217: 905-913, 2019 Feb.
Article
en En
| MEDLINE
| ID: mdl-30466059
ABSTRACT
Aflatoxin B1 (AFB1) is the major mycotoxin that contaminates aquafeeds and regarded as a causative agent in illnesses and the mortality of aquacultural species. However, the effects of AFB1 on developing fish and associated toxic mechanism are still unknown. This study examines the behavioral changes, neuronal morphology and gene expression in zebrafish embryos and larvae upon exposure to aflatoxin solutions. Treatment of 6â¯h post fertilization (hpf) embryos with AFB1 at 15-75â¯ng/mL significantly changed the swimming patterns of seven days post-fertilization (dpf) zebrafish larvae. Larvae in the 15â¯ng/mL group demonstrated a hypolocomotor activity in free swimming, but hyperlocomotion was observed in the larvae exposed to 30-75â¯ng/mL AFB1. AFB1 at 75â¯ng/mL also significantly reduced the startle response of 7 dpf larvae after tapping stimulus. Exposure to AFB1 resulted in an aberrant morphology of trigeminal ganglion and hindbrain neurons in transgenic embryos (HuCeGFP); this finding was supported by acetylated alpha-tubulin staining in wild-type fish. Additionally, AFB1 altered the levels of neurotoxic markers, including gfap and huC. The transcriptomic profile of AFB1-treated embryos revealed several differentially expressed genes that are related to neuroactivity and neurogenesis. PCR analysis verified that AFB1 significantly down-regulated the expression of ngfa and atp1b1b genes and increased that of prtga gene. The results herein indicate the toxicological impacts of AFB1 on the behaviors and neurodevelopment of fish in the early embryonic stage. Disruption of neural formation and synapse dysfunction may be responsible for the behavioral alteration.
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Bases de datos:
MEDLINE
Asunto principal:
Pez Cebra
/
Aflatoxina B1
Límite:
Animals
Idioma:
En
Revista:
Chemosphere
Año:
2019
Tipo del documento:
Article
País de afiliación:
Taiwán