Interspecies transmission to bovinized transgenic mice uncovers new features of a CH1641-like scrapie isolate.
Vet Res
; 49(1): 116, 2018 Nov 28.
Article
en En
| MEDLINE
| ID: mdl-30486902
ABSTRACT
In animal prion diseases, including bovine spongiform encephalopathy (BSE) in cattle, chronic wasting disease in cervids, and scrapie in sheep and goats, a disease-associated isoform of prion protein (PrPd) accumulates in the brains of affected animals. Although the CH1641 scrapie isolate was experimentally established in the UK, a few natural CH1641-like scrapie cases have been reported in France and the UK. The molecular mass of the unglycosylated protease-resistant core of PrPd (PrPres) is known to be similar between CH1641-like scrapie and experimental BSE in sheep. We previously established an experimental CH1641-like scrapie isolate (Sh294) from a natural classical scrapie case. Here, we demonstrated that the Sh294 isolate was independent of both classical and atypical BSEs by cross-species transmission to bovine PrP overexpressing (TgBoPrP) mice and wild-type mice. Interestingly, we found that the Sh294 isolate altered its host range by the transmission to TgBoPrP mice, and we succeeded in the first stable reproduction of CH1641-like scrapie specific PrPres banding patterns with the ~12-kDa small C-terminal fragment in wild-type mice. This study provides new insight into the relationship between CH1641-like scrapie isolates and BSEs. In addition, interspecies transmission models such as we have demonstrated here could be a great help to investigate the origin and host range of animal prions.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Scrapie
/
Priones
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Encefalopatía Espongiforme Bovina
/
Enfermedades por Prión
/
Proteínas PrPSc
Límite:
Animals
Idioma:
En
Revista:
Vet Res
Asunto de la revista:
MEDICINA VETERINARIA
Año:
2018
Tipo del documento:
Article
País de afiliación:
Japón