A unified model for microtubule rescue.
Mol Biol Cell
; 30(6): 753-765, 2019 03 15.
Article
en En
| MEDLINE
| ID: mdl-30672721
ABSTRACT
How microtubules transition from depolymerization to polymerization, known as rescue, is poorly understood. Here we examine two models for rescue 1) an "end-driven" model in which the depolymerizing end stochastically switches to a stable state; and 2) a "lattice-driven" model in which rescue sites are integrated into the microtubule before depolymerization. We test these models using a combination of computational simulations and in vitro experiments with purified tubulin. Our findings support the "lattice-driven" model by identifying repeated rescue sites in microtubules. In addition, we discover an important role for divalent cations in determining the frequency and location of rescue sites. We use "wash-in" experiments to show that divalent cations inhibit rescue during depolymerization, but not during polymerization. We propose a unified model in which rescues are driven by embedded rescue sites in microtubules, but the activity of these sites is influenced by changes in the depolymerizing ends.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Microtúbulos
Tipo de estudio:
Health_economic_evaluation
/
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Mol Biol Cell
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2019
Tipo del documento:
Article