Activity of base analogues (5-fluorouracil, 5-flucytosine) against planktonic cells and mature biofilm of Candida yeast. Effect of combination with folinic acid.
J Mycol Med
; 29(2): 147-153, 2019 Jun.
Article
en En
| MEDLINE
| ID: mdl-31023592
ABSTRACT
BACKGROUND:
The increasing number of Candida infections, especially those caused by non-C. albicans species and resistant strains, is a serious medical problem.OBJECTIVES:
In this study, the antifungal activity of base analogues, 5-flucytosine (5-FC) and 5-fluorouracil (5-FU), was tested against planktonic cells as well as against mature biofilm.METHODS:
Tests were performed according the EUCAST methodology. Antibiofilm effectiveness of tested drugs was determined by the crystal violet staining method. The cytotoxicity assays was performed according to the ISO 10993-5 norm.RESULTS:
5-FC and 5-FU were effective against fifteen fluconazole resistant Candida glabrata strains with an average minimal inhibitory concentration (MIC) of 0.152mg/L and 0.39mg/L, respectively. Folinic acid (folinate- e.g., leucovorin) is a common drug used in oncology simultaneously with 5-FU. In our tests folinate was able to lower MIC for 5-FC from 0.152 to 0.058mg/L (P<0.05). In the biofilm assay 5-FU and 5-FC alone did not induce any changes in the biomass of mature biofilm. Addition of folinate to each base analogue resulted in up to 90% reduction of biomass. Viability tests show that a concentration of 64mg/L of 5-FC and 5-FU supplemented with folinate can be fungicidal against mature biofilms of some Candida isolates. No cytotoxic effect was found for combination of FOL and 5-FC.CONCLUSION:
Therapy of 5-FU+folinate is well known in cancer treatment, in this study we reveal the beneficial effect of folinate on antifungal activity of 5-FC as well as the antifungal potential of 5-FU+folinate.Palabras clave
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Bases de datos:
MEDLINE
Asunto principal:
Leucovorina
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Biopelículas
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Candida glabrata
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Flucitosina
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Fluorouracilo
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Antifúngicos
Idioma:
En
Revista:
J Mycol Med
Año:
2019
Tipo del documento:
Article