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Listeria innocua Dps as a nanoplatform for bioluminescence based photodynamic therapy utilizing Gaussia princeps luciferase and zinc protoporphyrin IX.
Al-Ani, Ali W; Zhang, Lei; Ferreira, Lenny; Turyanska, Lyudmila; Bradshaw, Tracey D; Thomas, Neil R.
Afiliación
  • Al-Ani AW; Centre for Biomolecular Sciences, School of Chemistry, The University of Nottingham, Nottingham, UK.
  • Zhang L; Centre for Biomolecular Sciences, School of Chemistry, The University of Nottingham, Nottingham, UK.
  • Ferreira L; Centre for Biomolecular Sciences, School of Chemistry, The University of Nottingham, Nottingham, UK.
  • Turyanska L; School of Physics & Astronomy, The University of Nottingham, University Park, Nottingham, UK; and School of Chemistry, University of Lincoln, UK.
  • Bradshaw TD; Centre for Biomolecular Sciences, School of Pharmacy, The University of Nottingham, University Park, Nottingham, UK.
  • Thomas NR; Centre for Biomolecular Sciences, School of Chemistry, The University of Nottingham, Nottingham, UK. Electronic address: neil.thomas@nottingham.ac.uk.
Nanomedicine ; 20: 102005, 2019 08.
Article en En | MEDLINE | ID: mdl-31048084
Listeria innocua DNA binding protein from starved cells (LiDps) belongs to the ferritin family and provides a promising self-assembling spherical 12-mer protein scaffold for the generation of functional nanomaterials. We report the creation of a Gaussia princeps luciferase (Gluc)-LiDps fusion protein, with chemical conjugation of Zinc (II)-protoporphyrin IX (ZnPP) to lysine residues on the fusion protein (giving Gluc-LiDps-ZnPP). The Gluc-LiDps-ZnPP conjugate is shown to generate reactive oxygen species (ROS) via Bioluminescence Resonance Energy Transfer (BRET) between the Gluc (470-490 nm) and ZnPP. In vitro, Gluc-LiDps-ZnPP is efficiently taken up by tumorigenic cells (SKBR3 and MDA-MB-231 breast cancer cells). In the presence of coelenterazine, this construct inhibits the proliferation of SKBR3 due to elevated ROS levels. Following exposure to Gluc-LiDps-ZnPP, migration of surviving SKBR3 cells is significantly suppressed. These results demonstrate the potential of the Gluc-LiDps-ZnPP conjugate as a platform for future development of an anticancer photodynamic therapy agent.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Fotoquimioterapia / Protoporfirinas / Copépodos / Nanopartículas / Listeria / Luciferasas / Mediciones Luminiscentes Límite: Animals / Humans Idioma: En Revista: Nanomedicine Asunto de la revista: BIOTECNOLOGIA Año: 2019 Tipo del documento: Article

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Fotoquimioterapia / Protoporfirinas / Copépodos / Nanopartículas / Listeria / Luciferasas / Mediciones Luminiscentes Límite: Animals / Humans Idioma: En Revista: Nanomedicine Asunto de la revista: BIOTECNOLOGIA Año: 2019 Tipo del documento: Article