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Tissue patrol by resident memory CD8+ T cells in human skin.
Dijkgraaf, Feline E; Matos, Tiago R; Hoogenboezem, Mark; Toebes, Mireille; Vredevoogd, David W; Mertz, Marjolijn; van den Broek, Bram; Song, Ji-Ying; Teunissen, Marcel B M; Luiten, Rosalie M; Beltman, Joost B; Schumacher, Ton N.
Afiliación
  • Dijkgraaf FE; Division of Molecular Oncology and Immunology, Oncode Institute, The Netherlands Cancer Institute, Amsterdam, the Netherlands.
  • Matos TR; Department of Dermatology and Netherlands Institute for Pigment Disorders, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, the Netherlands.
  • Hoogenboezem M; Research Facility, Sanquin Amsterdam, Amsterdam, the Netherlands.
  • Toebes M; Division of Molecular Oncology and Immunology, Oncode Institute, The Netherlands Cancer Institute, Amsterdam, the Netherlands.
  • Vredevoogd DW; Division of Molecular Oncology and Immunology, Oncode Institute, The Netherlands Cancer Institute, Amsterdam, the Netherlands.
  • Mertz M; BioImaging Facility, The Netherlands Cancer Institute, Amsterdam, the Netherlands.
  • van den Broek B; BioImaging Facility, The Netherlands Cancer Institute, Amsterdam, the Netherlands.
  • Song JY; Animal Pathology, The Netherlands Cancer Institute, Amsterdam, the Netherlands.
  • Teunissen MBM; Department of Dermatology and Netherlands Institute for Pigment Disorders, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, the Netherlands.
  • Luiten RM; Department of Dermatology and Netherlands Institute for Pigment Disorders, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, the Netherlands.
  • Beltman JB; Division of Drug Discovery and Safety, Leiden Academic Centre for Drug Research, Leiden University, Leiden, the Netherlands.
  • Schumacher TN; Division of Molecular Oncology and Immunology, Oncode Institute, The Netherlands Cancer Institute, Amsterdam, the Netherlands. t.schumacher@nki.nl.
Nat Immunol ; 20(6): 756-764, 2019 06.
Article en En | MEDLINE | ID: mdl-31110315
Emerging data show that tissue-resident memory T (TRM) cells play an important protective role at murine and human barrier sites. TRM cells in the epidermis of mouse skin patrol their surroundings and rapidly respond when antigens are encountered. However, whether a similar migratory behavior is performed by human TRM cells is unclear, as technology to longitudinally follow them in situ has been lacking. To address this issue, we developed an ex vivo culture system to label and track T cells in fresh skin samples. We validated this system by comparing in vivo and ex vivo properties of murine TRM cells. Using nanobody labeling, we subsequently demonstrated in human ex vivo skin that CD8+ TRM cells migrated through the papillary dermis and the epidermis, below sessile Langerhans cells. Collectively, this work allows the dynamic study of resident immune cells in human skin and provides evidence of tissue patrol by human CD8+ TRM cells.
Asunto(s)

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Piel / Linfocitos T CD8-positivos / Memoria Inmunológica Límite: Animals / Humans Idioma: En Revista: Nat Immunol Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Países Bajos

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Piel / Linfocitos T CD8-positivos / Memoria Inmunológica Límite: Animals / Humans Idioma: En Revista: Nat Immunol Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Países Bajos