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Comparison between MALDI-TOF MS and MicroScan in the identification of emerging and multidrug resistant yeasts in a fourth-level hospital in Bogotá, Colombia.
Ceballos-Garzón, Andrés; Cortes, Gloria; Morio, Florent; Zamora-Cruz, Edna L; Linares, Melva Y; Ariza, Beatriz E; Valderrama, Sandra L; Garzón, Javier R; Alvarez-Moreno, Carlos A; Le Pape, Patrice; Parra-Giraldo, Claudia M.
Afiliación
  • Ceballos-Garzón A; Departamento de Microbiología, Facultad de Ciencias, Unidad de Proteomica y Micosis Humanas, Grupo de Enfermedades Infecciosas, Pontificia Universidad Javeriana, Bogotá, Colombia.
  • Cortes G; Grupo de Investigación en Enfermedades Infecciosas, Hospital Universitario San Ignacio, Bogotá, Colombia.
  • Morio F; Department of Parasitology and Medical Mycology of the University of Nantes, Nantes Atlantique Universities, Faculty of Pharmacy, Nantes, France.
  • Zamora-Cruz EL; Laboratorio Clínico, Área de Microbiología, Hospital Universitario San Ignacio, Bogotá, Colombia.
  • Linares MY; Grupo de Investigación en Enfermedades Infecciosas, Hospital Universitario San Ignacio, Bogotá, Colombia.
  • Ariza BE; Department of Parasitology and Medical Mycology of the University of Nantes, Nantes Atlantique Universities, Faculty of Pharmacy, Nantes, France.
  • Valderrama SL; Departamento de Microbiología, Facultad de Ciencias, Unidad de Proteomica y Micosis Humanas, Grupo de Enfermedades Infecciosas, Pontificia Universidad Javeriana, Bogotá, Colombia.
  • Garzón JR; Departamento de Microbiología, Facultad de Ciencias, Unidad de Proteomica y Micosis Humanas, Grupo de Enfermedades Infecciosas, Pontificia Universidad Javeriana, Bogotá, Colombia.
  • Alvarez-Moreno CA; Grupo de Investigación en Enfermedades Infecciosas, Hospital Universitario San Ignacio, Bogotá, Colombia.
  • Le Pape P; Laboratorio Clínico, Área de Microbiología, Hospital Universitario San Ignacio, Bogotá, Colombia.
  • Parra-Giraldo CM; Grupo de Investigación en Enfermedades Infecciosas, Hospital Universitario San Ignacio, Bogotá, Colombia.
BMC Microbiol ; 19(1): 106, 2019 05 23.
Article en En | MEDLINE | ID: mdl-31122184
BACKGROUND: The introduction of MALDI-TOF MS in the clinical microbiology laboratory has modified the approaches for the identification of fungi. Thanks to this tool, it is possible to identify cryptic species, which possess critical susceptibility patterns. Clinical strains were identified using the MicroScan and MALDI-TOF MS systems. Discrepant results from both methods were investigated using ITS rDNA barcoding. Finally, these isolates were also tested for in vitro susceptibility. RESULTS: The percentage of agreement between both methods to 498 yeast isolates was of 93.6% (32 discrepant isolates). The concordance of ITS sequencing with MALDI-TOF MS was higher (99%) than that of MicroScan (94%). Several of these discordant yeasts displayed high MICs for antifungal agents. CONCLUSIONS: Our study highlights the need of the MS and molecular approaches such as MALDI-TOF MS and ITS rDNA barcoding for the correct identification of emerging or cryptic yeast species; besides, some of these could be multidrug resistant. This work was the first experience in the implementation of the MALDI-TOF MS technology in Colombia. We found the first uncommon yeasts including Candida auris and we could identify Trichosporon faecalis. Our work highlights a clear necessity of an accurate yeast identification as a much more pertinent technique than the susceptibility profiles, because the most unusual yeasts exhibit resistance profiles to the few available antifungals.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Levaduras / ADN Ribosómico / Farmacorresistencia Fúngica Múltiple / Micosis Tipo de estudio: Diagnostic_studies Límite: Humans País/Región como asunto: America do sul / Colombia Idioma: En Revista: BMC Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Colombia

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Levaduras / ADN Ribosómico / Farmacorresistencia Fúngica Múltiple / Micosis Tipo de estudio: Diagnostic_studies Límite: Humans País/Región como asunto: America do sul / Colombia Idioma: En Revista: BMC Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Colombia