Chemical constituents, antibacterial activity and mechanism of Paeonia suffruticosa Andr. buds extract against Staphylococcus aureus and Escherichia coli O157:H7.
Nat Prod Res
; 35(6): 1005-1009, 2021 Mar.
Article
en En
| MEDLINE
| ID: mdl-31135213
ABSTRACT
Sixteen chemical constituents of Paeonia suffruticosa Andr. buds extract (PSABE) were identified by UHPLC-PDA-Q/TOF-MS, belonging to phenolic acids, flavonoids, monoterpene glycosides and gallotannins. PSABE exhibited significant antibacterial activity against six tested microorganisms. Particularly, it showed the most efficient antibacterial effect against Staphylococcus aureus and Escherichia coli O157H7, which the minimum inhibition concentration (MIC) and minimum bactericide concentration (MBC) both were 1.56 mg/mL and 6.25 mg/mL, respectively. The results showed that PSABE induced obvious alterations in membrane fatty acid composition of S. aureus and E. coli O157H7, such as the decrease of unsaturated fatty acids, leading to the reduce of membrane fluidity. Membrane integrity was destroyed and cell morphology was obviously changed with PSABE. Furthermore, the transcription level of virulence factors was inhibited in the presence of PSABE. These results indicated that PSABE mainly exerted antibacterial effect by damaging cell membrane and inhibiting transcription level of virulence factors.[Figure see text].
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Staphylococcus aureus
/
Extractos Vegetales
/
Escherichia coli O157
/
Paeonia
/
Antibacterianos
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Nat Prod Res
Año:
2021
Tipo del documento:
Article
País de afiliación:
China