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A novel synthetic-genetic-array-based yeast one-hybrid system for high discovery rate and short processing time.
Yeh, Chung-Shu; Wang, Zhifeng; Miao, Fang; Ma, Hongyan; Kao, Chung-Ting; Hsu, Tzu-Shu; Yu, Jhong-He; Hung, Er-Tsi; Lin, Chia-Chang; Kuan, Chen-Yu; Tsai, Ni-Chiao; Zhou, Chenguang; Qu, Guan-Zheng; Jiang, Jing; Liu, Guifeng; Wang, Jack P; Li, Wei; Chiang, Vincent L; Chang, Tien-Hsien; Lin, Ying-Chung Jimmy.
Afiliación
  • Yeh CS; Genomics Research Center, Academia Sinica, Taipei 11529, Taiwan.
  • Wang Z; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China.
  • Miao F; Department of Life Sciences and Institute of Plant Biology, College of Life Science, National Taiwan University, Taipei 10617, Taiwan.
  • Ma H; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China.
  • Kao CT; Department of Life Sciences and Institute of Plant Biology, College of Life Science, National Taiwan University, Taipei 10617, Taiwan.
  • Hsu TS; Department of Life Sciences and Institute of Plant Biology, College of Life Science, National Taiwan University, Taipei 10617, Taiwan.
  • Yu JH; Institute of Biomedical Informatics and Center for Systems and Synthetic Biology, National Yang-Ming University, Taipei 11221, Taiwan.
  • Hung ET; Department of Life Sciences and Institute of Plant Biology, College of Life Science, National Taiwan University, Taipei 10617, Taiwan.
  • Lin CC; Department of Life Sciences and Institute of Plant Biology, College of Life Science, National Taiwan University, Taipei 10617, Taiwan.
  • Kuan CY; Department of Life Sciences and Institute of Plant Biology, College of Life Science, National Taiwan University, Taipei 10617, Taiwan.
  • Tsai NC; Department of Life Sciences and Institute of Plant Biology, College of Life Science, National Taiwan University, Taipei 10617, Taiwan.
  • Zhou C; Department of Life Sciences and Institute of Plant Biology, College of Life Science, National Taiwan University, Taipei 10617, Taiwan.
  • Qu GZ; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China.
  • Jiang J; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China.
  • Liu G; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China.
  • Wang JP; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China.
  • Li W; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China.
  • Chiang VL; Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, North Carolina 27695, USA.
  • Chang TH; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China.
  • Lin YJ; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China.
Genome Res ; 29(8): 1343-1351, 2019 08.
Article en En | MEDLINE | ID: mdl-31186303
ABSTRACT
Eukaryotic gene expression is often tightly regulated by interactions between transcription factors (TFs) and their DNA cis targets. Yeast one-hybrid (Y1H) is one of the most extensively used methods to discover these interactions. We developed a high-throughput meiosis-directed yeast one-hybrid system using the Magic Markers of the synthetic genetic array analysis. The system has a transcription factor-DNA interaction discovery rate twice as high as the conventional diploid-mating approach and a processing time nearly one-tenth of the haploid-transformation method. The system also offers the highest accuracy in identifying TF-DNA interactions that can be authenticated in vivo by chromatin immunoprecipitation. With these unique features, this meiosis-directed Y1H system is particularly suited for constructing novel and comprehensive genome-scale gene regulatory networks for various organisms.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Factores de Transcripción / ADN / Técnicas del Sistema de Dos Híbridos / Análisis por Micromatrices Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Genome Res Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2019 Tipo del documento: Article País de afiliación: Taiwán
Texto completo
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Factores de Transcripción / ADN / Técnicas del Sistema de Dos Híbridos / Análisis por Micromatrices Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Genome Res Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2019 Tipo del documento: Article País de afiliación: Taiwán