Detection of synergistic effect of superoxide dismutase and jujubosides on scavenging superoxide anion radical by capillary electrophoresis.
Biomed Chromatogr
; 33(10): e4630, 2019 Oct.
Article
en En
| MEDLINE
| ID: mdl-31243785
ABSTRACT
A new capillary electrophoresis method was developed to study the synergistic effect of superoxide dismutase and jujuboside A or B on scavenging superoxide anion radical in serum matrix respectively, in which superoxide anion radical was generated from pyrogallol autoxidation. The electrophoresis conditions, and the factors affecting the productive rate of purpurogallin, such as pyrogallol autoxidation product and the activity of superoxide dismutase, were optimized. Under optimal conditions, the content of superoxide dismutase in Gibco newborn calf serum was 7.06 mg/L, RSD was 2.01% and the average recovery was 98.4%. The values of IC50 for jujuboside A and B in the serum matrix were 157.67 and 31.60 mg/L respectively, and they both had synergy on scavenging superoxide anion radical with superoxide dismutase, but there was no the dose-dependency on this synergy.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Saponinas
/
Superóxido Dismutasa
/
Superóxidos
/
Electroforesis Capilar
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Revista:
Biomed Chromatogr
Año:
2019
Tipo del documento:
Article
País de afiliación:
China