Fine mapping of a silique length- and seed weight-related gene in Brassica napus.
Theor Appl Genet
; 132(11): 2985-2996, 2019 Nov.
Article
en En
| MEDLINE
| ID: mdl-31321475
ABSTRACT
KEY MESSAGE Using microarray analysis combined with map-based cloning, a major locus positively regulating SL and SW was mapped to a 98.47 kb interval on A09 in rapeseed. In rapeseed, seed yield is closely associated with silique-related traits such as silique length (SL) and seed weight (SW). Previously identified quantitative trait loci (QTLs) revealed that SL and SW are complex traits and many QTLs overlap. However, the genetic characterization of the association between SL and SW is poorly understood. In the present study, a BC3F3 near isogenic line developed from a short silique plant and the long silique cultivar 'ZS11' was analyzed to identify the locus related to SL. Map-based cloning indicated that a major locus acting as a single Mendelian factor was mapped to a 98.47 kb region on chromosome A09. BLAST analysis and DNA sequencing showed SNP variations and a fragment replacement in the upstream region of the candidate gene BnaA09g55530D may alter gene expression and influence SL. The results showed that this SL locus may also positively affect SW as well as in the 186 rapeseed accessions identified by the associated markers. Therefore, selecting plants with appropriate SL and developing functional markers for the associated gene could play important roles in the molecular breeding of high-yield rapeseed varieties.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Semillas
/
Mapeo Cromosómico
/
Genes de Plantas
/
Brassica napus
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Theor Appl Genet
Año:
2019
Tipo del documento:
Article
País de afiliación:
China