The effects of photodynamic therapy with blue light and papain-based gel associated with Urucum, on collagen and fibroblasts: a spectroscopic and cytotoxicity analysis.
Lasers Med Sci
; 35(3): 767-775, 2020 Apr.
Article
en En
| MEDLINE
| ID: mdl-31407100
ABSTRACT
Papacarie Duo™ is clinically used and has proven effectiveness; however, it is necessary to improve its antimicrobial action. The combined treatment of Papacarie Duo™ with Urucum (Bixa Orellana) could create a potential tool for dental caries treatment; its extract obtained from the seeds' pericarp contains a water-soluble primary pigment (cis-bixin) with smaller amounts of other carotenoids. The dicarboxylic acid salts of cis-norbixin and trans-norbixin occur in heated alkaline solutions. To analyze the absorption spectra and cytotoxicity (with human dermal fibroblasts) in different concentrations of Urucum, associated or not with Papacarie Duo™, we performed this in vitro study. The effects of pure Urucum, Papacarie Duo™, and PapaUrucum™ on the microstructure of collagen were also analyzed. The application of papain-based gel with Urucum did not present cytotoxicity, its exhibit UV absorption spectrum peak around 460 ± 20 nm. Also, it showed that the compound used did not alter the chemical structure of collagen. Consequently, this product could be used as a chemomechanical method to remove dentin caries as well as being a potential product for antimicrobial photodynamic therapy (aPDT) application.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Fotoquimioterapia
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Análisis Espectral
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Papaína
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Colágeno
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Bixaceae
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Fibroblastos
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Geles
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Luz
Tipo de estudio:
Risk_factors_studies
Límite:
Humans
Idioma:
En
Revista:
Lasers Med Sci
Asunto de la revista:
BIOTECNOLOGIA
/
RADIOLOGIA
Año:
2020
Tipo del documento:
Article
País de afiliación:
Estados Unidos