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Enhanced In Vivo Delivery of Stem Cells using Microporous Annealed Particle Scaffolds.
Koh, Jaekyung; Griffin, Donald R; Archang, Maani M; Feng, An-Chieh; Horn, Thomas; Margolis, Michael; Zalazar, David; Segura, Tatiana; Scumpia, Philip O; Di Carlo, Dino.
Afiliación
  • Koh J; Department of Bioengineering, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
  • Griffin DR; Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, 22904, USA.
  • Archang MM; Department of Bioengineering, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
  • Feng AC; Department of Microbiology, Immunology, and Molecular Genetics, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
  • Horn T; Department of Bioengineering, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
  • Margolis M; Department of Bioengineering, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
  • Zalazar D; Department of Bioengineering, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
  • Segura T; Department of Biomedical Engineering, Neurology, Dermatology, Duke University, Durham, NC, 27708, USA.
  • Scumpia PO; Division of Dermatology, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
  • Di Carlo D; Department of Dermatology, VA Greater Los Angeles Healthcare System, Los Angeles, CA, 90073, USA.
Small ; 15(39): e1903147, 2019 09.
Article en En | MEDLINE | ID: mdl-31410986
ABSTRACT
Delivery to the proper tissue compartment is a major obstacle hampering the potential of cellular therapeutics for medical conditions. Delivery of cells within biomaterials may improve localization, but traditional and newer void-forming hydrogels must be made in advance with cells being added into the scaffold during the manufacturing process. Injectable, in situ cross-linking microporous scaffolds are recently developed that demonstrate a remarkable ability to provide a matrix for cellular proliferation and growth in vitro in three dimensions. The ability of these scaffolds to deliver cells in vivo is currently unknown. Herein, it is shown that mesenchymal stem cells (MSCs) can be co-injected locally with microparticle scaffolds assembled in situ immediately following injection. MSC delivery within a microporous scaffold enhances MSC retention subcutaneously when compared to cell delivery alone or delivery within traditional in situ cross-linked nanoporous hydrogels. After two weeks, endothelial cells forming blood vessels are recruited to the scaffold and cells retaining the MSC marker CD29 remain viable within the scaffold. These findings highlight the utility of this approach in achieving localized delivery of stem cells through an injectable porous matrix while limiting obstacles of introducing cells within the scaffold manufacturing process.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Células Madre / Andamios del Tejido Límite: Animals Idioma: En Revista: Small Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Células Madre / Andamios del Tejido Límite: Animals Idioma: En Revista: Small Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos