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Near-Infrared Optogenetic Genome Engineering Based on Photon-Upconversion Hydrogels.
Sasaki, Yoichi; Oshikawa, Mio; Bharmoria, Pankaj; Kouno, Hironori; Hayashi-Takagi, Akiko; Sato, Moritoshi; Ajioka, Itsuki; Yanai, Nobuhiro; Kimizuka, Nobuo.
Afiliación
  • Sasaki Y; Department of Chemistry and Biochemistry, Graduate School of Engineering, Center for Molecular Systems (CMS), Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka, 819-0395, Japan.
  • Oshikawa M; Center for Brain Integration Research (CBIR), Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.
  • Bharmoria P; Department of Chemistry and Biochemistry, Graduate School of Engineering, Center for Molecular Systems (CMS), Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka, 819-0395, Japan.
  • Kouno H; Department of Chemistry and Biochemistry, Graduate School of Engineering, Center for Molecular Systems (CMS), Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka, 819-0395, Japan.
  • Hayashi-Takagi A; Laboratory of Medical Neuroscience, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi-city, Gunma, 371-8512, Japan.
  • Sato M; PRESTO, JST, Honcho 4-1-8, Kawaguchi, Saitama, 332-0012, Japan.
  • Ajioka I; Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo, 153-8902, Japan.
  • Yanai N; Center for Brain Integration Research (CBIR), Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.
  • Kimizuka N; PRESTO, JST, Honcho 4-1-8, Kawaguchi, Saitama, 332-0012, Japan.
Angew Chem Int Ed Engl ; 58(49): 17827-17833, 2019 12 02.
Article en En | MEDLINE | ID: mdl-31544993
ABSTRACT
Photon upconversion (UC) from near-infrared (NIR) light to visible light has enabled optogenetic manipulations in deep tissues. However, materials for NIR optogenetics have been limited to inorganic UC nanoparticles. Herein, NIR-light-triggered optogenetics using biocompatible, organic TTA-UC hydrogels is reported. To achieve triplet sensitization even in highly viscous hydrogel matrices, a NIR-absorbing complex is covalently linked with energy-pooling acceptor chromophores, which significantly elongates the donor triplet lifetime. The donor and acceptor are solubilized in hydrogels formed from biocompatible Pluronic F127 micelles, and heat treatment endows the excited triplets in the hydrogel with remarkable oxygen tolerance. Combined with photoactivatable Cre recombinase technology, NIR-light stimulation successfully performs genome engineering resulting in the formation of dendritic-spine-like structures of hippocampal neurons.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Osmio / Perileno / Hidrogeles / Complejos de Coordinación / Colorantes Fluorescentes Idioma: En Revista: Angew Chem Int Ed Engl Año: 2019 Tipo del documento: Article País de afiliación: Japón
Texto completo
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XML
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Osmio / Perileno / Hidrogeles / Complejos de Coordinación / Colorantes Fluorescentes Idioma: En Revista: Angew Chem Int Ed Engl Año: 2019 Tipo del documento: Article País de afiliación: Japón