Moderate hyperoxia plays a protective role in lung bronchial epithelial cells.
Clin Invest Med
; 42(4): E28-E36, 2019 12 29.
Article
en En
| MEDLINE
| ID: mdl-31922706
ABSTRACT
PURPOSE:
Oxygen therapy is commonly used in clinical settings, but several problems may result from improper use. Oxygen poisoning involves the initiation of a series of inflammatory reactions. In this study, we compared the effects of moderate hyperoxia (40% O2) and extreme hyperoxia (85% O2) on pulmonary bronchial epithelial cells.METHODS:
Normal human tracheobronchial epithelium (NHBE) cells were exposed to hyperoxia (40% and 85%) for 24 hours, and their survival rates were determined by the colorimetic assay, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide). The protein and mRNA levels of RelA, RelB, ASK1, TNF-α and secretory component (SC) were detected by immunohistochemical staining, western blot, and real-time polymerase chain reaction.RESULTS:
The NHBE cell survival increased in the presence of moderate hyperoxia. RelA, RelB, ASK1, TNF-α and SC expressions were significantly higher in the 85% O2 group in comparison with the control group and the 40% O2 group. In the 40% O2 group, RelA, RelB, ASK1 and TNF-α were upregulated, but SC expression was not significantly different than that of the control group. However, compared with the 85% O2 group, SC expression was significantly lower in the 40% O2 group.CONCLUSION:
These results suggest that moderate hyperoxia promotes proliferation in NHBE cells and activates TNF-α and downstream ASK1. Then TNF-α activates NF-κB and SC to play a protective role.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Hiperoxia
/
Células Epiteliales
Límite:
Humans
Idioma:
En
Revista:
Clin Invest Med
Año:
2019
Tipo del documento:
Article
País de afiliación:
China