Bellymount enables longitudinal, intravital imaging of abdominal organs and the gut microbiota in adult Drosophila.
PLoS Biol
; 18(1): e3000567, 2020 01.
Article
en En
| MEDLINE
| ID: mdl-31986129
ABSTRACT
Cell- and tissue-level processes often occur across days or weeks, but few imaging methods can capture such long timescales. Here, we describe Bellymount, a simple, noninvasive method for longitudinal imaging of the Drosophila abdomen at subcellular resolution. Bellymounted animals remain live and intact, so the same individual can be imaged serially to yield vivid time series of multiday processes. This feature opens the door to longitudinal studies of Drosophila internal organs in their native context. Exploiting Bellymount's capabilities, we track intestinal stem cell lineages and gut microbial colonization in single animals, revealing spatiotemporal dynamics undetectable by previously available methods.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Vísceras
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Anatomía Transversal
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Drosophila
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Microscopía Intravital
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Microbioma Gastrointestinal
Tipo de estudio:
Observational_studies
Límite:
Animals
Idioma:
En
Revista:
PLoS Biol
Asunto de la revista:
BIOLOGIA
Año:
2020
Tipo del documento:
Article
País de afiliación:
Estados Unidos