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Robotic fluidic coupling and interrogation of multiple vascularized organ chips.
Novak, Richard; Ingram, Miles; Marquez, Susan; Das, Debarun; Delahanty, Aaron; Herland, Anna; Maoz, Ben M; Jeanty, Sauveur S F; Somayaji, Mahadevabharath R; Burt, Morgan; Calamari, Elizabeth; Chalkiadaki, Angeliki; Cho, Alexander; Choe, Youngjae; Chou, David Benson; Cronce, Michael; Dauth, Stephanie; Divic, Toni; Fernandez-Alcon, Jose; Ferrante, Thomas; Ferrier, John; FitzGerald, Edward A; Fleming, Rachel; Jalili-Firoozinezhad, Sasan; Grevesse, Thomas; Goss, Josue A; Hamkins-Indik, Tiama; Henry, Olivier; Hinojosa, Chris; Huffstater, Tessa; Jang, Kyung-Jin; Kujala, Ville; Leng, Lian; Mannix, Robert; Milton, Yuka; Nawroth, Janna; Nestor, Bret A; Ng, Carlos F; O'Connor, Blakely; Park, Tae-Eun; Sanchez, Henry; Sliz, Josiah; Sontheimer-Phelps, Alexandra; Swenor, Ben; Thompson, Guy; Touloumes, George J; Tranchemontagne, Zachary; Wen, Norman; Yadid, Moran; Bahinski, Anthony.
Afiliación
  • Novak R; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Ingram M; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Marquez S; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Das D; CFD Research Corporation, Huntsville, AL, USA.
  • Delahanty A; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Herland A; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Maoz BM; Division of Micro and Nanosystems, KTH Royal Institute of Technology, Stockholm, Sweden.
  • Jeanty SSF; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Somayaji MR; Disease Biophysics Group, Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA.
  • Burt M; Department of Biomedical Engineering and Sagol School of Neuroscience, Tel Aviv University, Tel Aviv, Israel.
  • Calamari E; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Chalkiadaki A; Emulate, Inc., Boston, MA, USA.
  • Cho A; CFD Research Corporation, Huntsville, AL, USA.
  • Choe Y; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Chou DB; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Cronce M; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Dauth S; CFD Research Corporation, Huntsville, AL, USA.
  • Divic T; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Fernandez-Alcon J; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Ferrante T; Department of Pathology, Massachusetts General Hospital, Boston, MA, USA.
  • Ferrier J; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • FitzGerald EA; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Fleming R; Disease Biophysics Group, Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA.
  • Jalili-Firoozinezhad S; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Grevesse T; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Goss JA; Emulate, Inc., Boston, MA, USA.
  • Hamkins-Indik T; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Henry O; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Hinojosa C; Disease Biophysics Group, Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA.
  • Huffstater T; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Jang KJ; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Kujala V; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Leng L; Department of Bioengineering and iBB-Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal.
  • Mannix R; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Milton Y; Disease Biophysics Group, Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA.
  • Nawroth J; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Nestor BA; Disease Biophysics Group, Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA.
  • Ng CF; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • O'Connor B; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Park TE; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Sanchez H; Emulate, Inc., Boston, MA, USA.
  • Sliz J; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Sontheimer-Phelps A; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Swenor B; Emulate, Inc., Boston, MA, USA.
  • Thompson G; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Touloumes GJ; Disease Biophysics Group, Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA.
  • Tranchemontagne Z; Emulate, Inc., Boston, MA, USA.
  • Wen N; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Yadid M; Emulate, Inc., Boston, MA, USA.
  • Bahinski A; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Nat Biomed Eng ; 4(4): 407-420, 2020 04.
Article en En | MEDLINE | ID: mdl-31988458
ABSTRACT
Organ chips can recapitulate organ-level (patho)physiology, yet pharmacokinetic and pharmacodynamic analyses require multi-organ systems linked by vascular perfusion. Here, we describe an 'interrogator' that employs liquid-handling robotics, custom software and an integrated mobile microscope for the automated culture, perfusion, medium addition, fluidic linking, sample collection and in situ microscopy imaging of up to ten organ chips inside a standard tissue-culture incubator. The robotic interrogator maintained the viability and organ-specific functions of eight vascularized, two-channel organ chips (intestine, liver, kidney, heart, lung, skin, blood-brain barrier and brain) for 3 weeks in culture when intermittently fluidically coupled via a common blood substitute through their reservoirs of medium and endothelium-lined vascular channels. We used the robotic interrogator and a physiological multicompartmental reduced-order model of the experimental system to quantitatively predict the distribution of an inulin tracer perfused through the multi-organ human-body-on-chips. The automated culture system enables the imaging of cells in the organ chips and the repeated sampling of both the vascular and interstitial compartments without compromising fluidic coupling.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Robótica / Técnicas de Cultivo de Célula / Microfluídica / Dispositivos Laboratorio en un Chip Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Nat Biomed Eng Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Robótica / Técnicas de Cultivo de Célula / Microfluídica / Dispositivos Laboratorio en un Chip Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Nat Biomed Eng Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos